Detailed information of TA system TA077470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 3485918..3486572
• Replicon: chromosome
• Accession: NZ_CP021804
• Organism: Sinorhizobium meliloti strain T073

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: CDO28_RS17120
• Protein ID: WP_003530010.1
• Coordinates: 3486384..3486572 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: CDO28_RS17115
• Protein ID: WP_010969659.1
• Coordinates: 3485918..3486379 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO28_RS17090	3482053..3482496	+	444	WP_003529999.1	TIGR02594 family protein	-
CDO28_RS17095	3482647..3483753	+	1107	WP_003530001.1	endonuclease	-
CDO28_RS17100	3483756..3484505	+	750	WP_010969660.1	NUDIX hydrolase	-
CDO28_RS17105	3484682..3485599	+	918	WP_003530005.1	PhzF family phenazine biosynthesis protein	-
CDO28_RS17115	3485918..3486379	-	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CDO28_RS17120	3486384..3486572	-	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDO28_RS17125	3486749..3487519	+	771	WP_010969658.1	hypothetical protein	-
CDO28_RS17130	3487700..3488671	+	972	WP_017265202.1	glycosyltransferase family 2 protein	-
CDO28_RS17135	3488696..3490006	-	1311	WP_088195594.1	HlyD family type I secretion periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T77470 WP_003530010.1 NZ_CP021804:c3486572-3486384 [Sinorhizobium meliloti]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T77470 NZ_CP021804:c3486572-3486384 [Sinorhizobium meliloti]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT77470 WP_010969659.1 NZ_CP021804:c3486379-3485918 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT77470 NZ_CP021804:c3486379-3485918 [Sinorhizobium meliloti]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0