Detailed information of TA system TA077462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 762430..763004
• Replicon: chromosome
• Accession: NZ_CP021804
• Organism: Sinorhizobium meliloti strain T073

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92QN4
• Locus tag: CDO28_RS04035
• Protein ID: WP_010969154.1
• Coordinates: 762639..763004 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92QN3
• Locus tag: CDO28_RS04030
• Protein ID: WP_010969155.1
• Coordinates: 762430..762645 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO28_RS04010	758356..759042	-	687	WP_010969157.1	ABC transporter ATP-binding protein	-
CDO28_RS04015	759060..760370	-	1311	WP_014529722.1	lipoprotein-releasing ABC transporter permease subunit	-
CDO28_RS04020	760391..761719	-	1329	WP_003531873.1	proline--tRNA ligase	-
CDO28_RS04025	762135..762362	+	228	WP_017264047.1	hypothetical protein	-
CDO28_RS04030	762430..762645	+	216	WP_010969155.1	ribbon-helix-helix protein, CopG family	Antitoxin
CDO28_RS04035	762639..763004	+	366	WP_010969154.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO28_RS04040	763091..763360	-	270	WP_014526683.1	DUF1467 family protein	-
CDO28_RS04045	763500..763904	-	405	WP_010969152.1	methylmalonyl-CoA epimerase	-
CDO28_RS04050	763963..765630	-	1668	WP_010969151.1	ribonuclease J	-
CDO28_RS04055	765658..766425	-	768	WP_003531861.1	biotin--[acetyl-CoA-carboxylase] ligase	-
CDO28_RS04060	766422..767864	-	1443	WP_014526682.1	NADH-quinone oxidoreductase subunit NuoN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13592.57 Da
• Isoelectric Point: 5.1787

>T77462 WP_010969154.1 NZ_CP021804:762639-763004 [Sinorhizobium meliloti]
MVGALFDTNILIDHLNAVPQAHKELDRFENRAISIITWMEVMVGADAELVEPTRRFLDGFETIALNDEIANRAVTLRRAH
RIKLPDAVIWATAQTAGRLLVTRNTKDFPADDPGIREPYAV

Nucleotide

• Length: 366 bp

>T77462 NZ_CP021804:762639-763004 [Sinorhizobium meliloti]
ATGGTAGGCGCACTGTTCGATACCAATATCCTTATCGATCATCTGAATGCGGTGCCTCAGGCGCACAAGGAACTTGACCG
TTTCGAAAACCGGGCCATCAGCATCATCACCTGGATGGAGGTGATGGTTGGAGCGGACGCCGAACTGGTTGAGCCGACCC
GGCGCTTCCTCGACGGTTTCGAAACGATCGCCTTGAACGATGAGATCGCCAACCGTGCGGTGACATTGCGCAGGGCGCAC
CGCATCAAGCTACCCGACGCGGTGATCTGGGCAACGGCGCAGACTGCCGGTCGTCTGCTCGTGACGCGCAATACCAAGGA
CTTTCCAGCCGACGACCCGGGGATACGCGAACCCTACGCCGTGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8381.51 Da
• Isoelectric Point: 8.6052

>AT77462 WP_010969155.1 NZ_CP021804:762430-762645 [Sinorhizobium meliloti]
MRALIDMNDTQVEALDTLAKRVRRSRAALIREAIDDYLNRHHREQIEDGFGLWGKRKVDGLAYQEKVRGEW

Nucleotide

• Length: 216 bp

>AT77462 NZ_CP021804:762430-762645 [Sinorhizobium meliloti]
ATGCGAGCCTTGATCGATATGAACGATACCCAGGTCGAAGCCCTAGATACCCTCGCCAAGCGGGTGCGCCGGTCCCGTGC
GGCTCTGATCCGCGAAGCGATCGACGACTATCTCAACCGTCATCATCGCGAGCAGATCGAAGATGGCTTCGGTTTGTGGG
GCAAGCGCAAAGTTGACGGTCTGGCTTATCAGGAGAAGGTGCGCGGCGAATGGTAG

Structures

Toxin

• AlphaFold DB: Q92QN4

Antitoxin

• AlphaFold DB: Q92QN3