Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4961342..4961883 Replicon chromosome
Accession NZ_CP021726
Organism Escherichia coli strain Combat11I9

Toxin (Protein)


Gene name yafQ Uniprot ID A0A246NT41
Locus tag CDC27_RS25000 Protein ID WP_000615977.1
Coordinates 4961342..4961620 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag CDC27_RS25005 Protein ID WP_000729709.1
Coordinates 4961623..4961883 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDC27_RS24985 4958924..4959502 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
CDC27_RS24990 4959708..4960475 + 768 WP_000333380.1 class II glutamine amidotransferase -
CDC27_RS24995 4960446..4961186 - 741 WP_001225679.1 murein L,D-transpeptidase -
CDC27_RS25000 4961342..4961620 - 279 WP_000615977.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CDC27_RS25005 4961623..4961883 - 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CDC27_RS25010 4962093..4962842 + 750 WP_072686764.1 C40 family peptidase -
CDC27_RS25015 4963018..4963515 + 498 WP_000006266.1 REP-associated tyrosine transposase RayT -
CDC27_RS25020 4963748..4965460 - 1713 Protein_4555 flagellar biosynthesis protein FlhA -
CDC27_RS25025 4965432..4966217 + 786 WP_072686765.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10842.67 Da        Isoelectric Point: 10.0702

>T77176 WP_000615977.1 NZ_CP021726:c4961620-4961342 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNILPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T77176 NZ_CP021726:c4961620-4961342 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATATTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT77176 WP_000729709.1 NZ_CP021726:c4961883-4961623 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT77176 NZ_CP021726:c4961883-4961623 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A246NT41

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References