Detailed information of TA system TA077172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3636750..3637381
• Replicon: chromosome
• Accession: NZ_CP021726
• Organism: Escherichia coli strain Combat11I9

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CDC27_RS18645
• Protein ID: WP_072686259.1
• Coordinates: 3637106..3637381 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q31VE4
• Locus tag: CDC27_RS18640
• Protein ID: WP_000593554.1
• Coordinates: 3636750..3637109 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC27_RS18615	3632882..3633826	+	945	WP_000947082.1	nickel ABC transporter permease subunit NikB	-
CDC27_RS18620	3633823..3634656	+	834	WP_072686260.1	nickel ABC transporter permease subunit NikC	-
CDC27_RS18625	3634656..3635420	+	765	WP_047644476.1	nickel import ATP-binding protein NikD	-
CDC27_RS18630	3635417..3636223	+	807	WP_032288306.1	nickel import ATP-binding protein NikE	-
CDC27_RS18635	3636229..3636630	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
CDC27_RS18640	3636750..3637109	-	360	WP_000593554.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CDC27_RS18645	3637106..3637381	-	276	WP_072686259.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDC27_RS18650	3637454..3638578	-	1125	WP_001314210.1	ABC transporter permease	-
CDC27_RS18655	3638578..3641313	-	2736	WP_072686258.1	ribosome-associated ATPase/putative transporter RbbA	-
CDC27_RS18660	3641310..3642377	-	1068	WP_000361517.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10166.87 Da
• Isoelectric Point: 10.7076

>T77172 WP_072686259.1 NZ_CP021726:c3637381-3637106 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T77172 NZ_CP021726:c3637381-3637106 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13359.25 Da
• Isoelectric Point: 4.8838

>AT77172 WP_000593554.1 NZ_CP021726:c3637109-3636750 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT77172 NZ_CP021726:c3637109-3636750 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A6H2GM94