Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 185921..186444 Replicon plasmid tig00000793
Accession NZ_CP021720
Organism Escherichia coli strain AR_0128

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag AM375_RS01995 Protein ID WP_015632464.1
Coordinates 185921..186226 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag AM375_RS02000 Protein ID WP_015632465.1
Coordinates 186226..186444 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM375_RS01950 180951..181379 - 429 WP_015632456.1 plasmid partitioning/stability family protein -
AM375_RS01955 181372..182352 - 981 WP_015632457.1 partitioning protein -
AM375_RS01960 182713..182895 + 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
AM375_RS01965 182921..183364 + 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
AM375_RS01970 183570..183692 - 123 Protein_200 transcriptional regulator -
AM375_RS01975 183706..183933 - 228 WP_016162072.1 hypothetical protein -
AM375_RS01980 184363..184620 + 258 WP_016162071.1 helix-turn-helix domain-containing protein -
AM375_RS01985 184824..185390 - 567 WP_015632462.1 hypothetical protein -
AM375_RS01990 185432..185776 - 345 WP_015632463.1 hypothetical protein -
AM375_RS01995 185921..186226 - 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
AM375_RS02000 186226..186444 - 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM375_RS02005 186630..187655 + 1026 WP_015632466.1 IS110 family transposase -
AM375_RS02010 187950..188435 + 486 Protein_208 IS5 family transposase -
AM375_RS02015 188599..189030 + 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
AM375_RS02020 189030..190301 + 1272 WP_016162068.1 Y-family DNA polymerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 mrkJ / mrkF / htpB 1..216378 216378


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T77126 WP_015632464.1 NZ_CP021720:c186226-185921 [Escherichia coli]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

>T77126 NZ_CP021720:c186226-185921 [Escherichia coli]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT77126 WP_015632465.1 NZ_CP021720:c186444-186226 [Escherichia coli]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp

>AT77126 NZ_CP021720:c186444-186226 [Escherichia coli]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References