Detailed information of TA system TA077123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2899..3556
• Replicon: plasmid tig00000792
• Accession: NZ_CP021719
• Organism: Escherichia coli strain AR_0128

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AM375_RS00040
• Protein ID: WP_000270043.1
• Coordinates: 2899..3249 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM375_RS00045
• Protein ID: WP_000124640.1
• Coordinates: 3254..3556 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM375_RS00010	458..793	+	336	WP_000683477.1	hypothetical protein	-
AM375_RS00015	808..1278	-	471	WP_001281821.1	hypothetical protein	-
AM375_RS00020	1271..1642	-	372	WP_000516918.1	hypothetical protein	-
AM375_RS00025	1653..1847	-	195	WP_000343597.1	hypothetical protein	-
AM375_RS00035	2188..2736	-	549	WP_001061573.1	hypothetical protein	-
AM375_RS00040	2899..3249	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM375_RS00045	3254..3556	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AM375_RS00050	3583..3876	-	294	WP_001239998.1	hypothetical protein	-
AM375_RS00055	3965..4237	-	273	WP_001043046.1	HU family DNA-binding protein	-
AM375_RS00060	4295..4822	-	528	WP_004201083.1	thermonuclease family protein	-
AM375_RS28305	4825..5130	-	306	WP_171820680.1	hypothetical protein	-
AM375_RS00070	5042..5899	-	858	WP_001167036.1	hypothetical protein	-
AM375_RS00075	5886..6116	-	231	WP_000972665.1	hypothetical protein	-
AM375_RS00080	6116..6634	-	519	WP_000210757.1	nitrite reductase	-
AM375_RS00085	6631..7077	-	447	WP_000919343.1	hypothetical protein	-
AM375_RS00090	7077..7436	-	360	WP_000422769.1	hypothetical protein	-
AM375_RS00095	7494..7922	-	429	WP_000591076.1	hypothetical protein	-
AM375_RS00100	7956..8426	-	471	Protein_18	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	rmtC / sul1 / qacE / aac(6')-Ib / blaCMY-6	-	1..128094	128094

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T77123 WP_000270043.1 NZ_CP021719:2899-3249 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T77123 NZ_CP021719:2899-3249 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT77123 WP_000124640.1 NZ_CP021719:3254-3556 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT77123 NZ_CP021719:3254-3556 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9