Detailed information of TA system TA077097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 152786..153443
• Replicon: plasmid tig00000001_p1
• Accession: NZ_CP021709
• Organism: Klebsiella pneumoniae strain AR_0143

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AM390_RS27930
• Protein ID: WP_000270043.1
• Coordinates: 153093..153443 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM390_RS27925
• Protein ID: WP_000124640.1
• Coordinates: 152786..153088 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM390_RS27875	148420..148848	+	429	WP_000591076.1	hypothetical protein	-
AM390_RS27880	148906..149265	+	360	WP_000422769.1	hypothetical protein	-
AM390_RS27885	149265..149711	+	447	WP_000919343.1	hypothetical protein	-
AM390_RS27890	149708..150226	+	519	WP_000210757.1	nitrite reductase	-
AM390_RS27895	150226..150456	+	231	WP_000972665.1	hypothetical protein	-
AM390_RS27900	150443..151300	+	858	WP_001167036.1	hypothetical protein	-
AM390_RS31315	151212..151517	+	306	WP_171820680.1	hypothetical protein	-
AM390_RS27910	151520..152047	+	528	WP_004201083.1	thermonuclease family protein	-
AM390_RS27915	152105..152377	+	273	WP_001043046.1	HU family DNA-binding protein	-
AM390_RS27920	152466..152759	+	294	WP_001239998.1	hypothetical protein	-
AM390_RS27925	152786..153088	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AM390_RS27930	153093..153443	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM390_RS27935	153606..154154	+	549	WP_001061573.1	hypothetical protein	-
AM390_RS27945	154495..154689	+	195	WP_000343597.1	hypothetical protein	-
AM390_RS27950	154700..155071	+	372	WP_000516918.1	hypothetical protein	-
AM390_RS27955	155064..155534	+	471	WP_001281821.1	hypothetical protein	-
AM390_RS27960	155549..155884	-	336	WP_000683477.1	hypothetical protein	-
AM390_RS27965	155981..156469	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
AM390_RS27970	156472..156969	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-4 / blaNDM-1 / sul1 / armA / msr(E) / mph(E)	-	1..186758	186758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T77097 WP_000270043.1 NZ_CP021709:c153443-153093 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T77097 NZ_CP021709:c153443-153093 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT77097 WP_000124640.1 NZ_CP021709:c153088-152786 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT77097 NZ_CP021709:c153088-152786 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9