Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2170512..2171137 Replicon chromosome
Accession NZ_CP021685
Organism Klebsiella pneumoniae strain AR_0146

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag AM393_RS10760 Protein ID WP_002882817.1
Coordinates 2170754..2171137 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag AM393_RS10755 Protein ID WP_004150355.1
Coordinates 2170512..2170754 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM393_RS10730 2166503..2167102 + 600 WP_004151865.1 glucose-1-phosphatase -
AM393_RS10735 2167096..2167956 + 861 WP_002882807.1 virulence factor BrkB family protein -
AM393_RS10740 2167953..2168390 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
AM393_RS10745 2168435..2169376 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
AM393_RS10750 2169390..2170307 - 918 WP_002882812.1 alpha/beta hydrolase -
AM393_RS10755 2170512..2170754 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
AM393_RS10760 2170754..2171137 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
AM393_RS10765 2171311..2172240 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
AM393_RS10770 2172237..2172872 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
AM393_RS10775 2172869..2173771 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T76972 WP_002882817.1 NZ_CP021685:2170754-2171137 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T76972 NZ_CP021685:2170754-2171137 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT76972 WP_004150355.1 NZ_CP021685:2170512-2170754 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT76972 NZ_CP021685:2170512-2170754 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References