Detailed information of TA system TA076878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1245916..1246477
• Replicon: chromosome
• Accession: NZ_CP021644
• Organism: Glaesserella parasuis 29755

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A143CE91
• Locus tag: B4U42_RS06785
• Protein ID: WP_005712614.1
• Coordinates: 1245916..1246194 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: B4U42_RS06790
• Protein ID: WP_005712616.1
• Coordinates: 1246205..1246477 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4U42_RS06770	1241701..1243029	-	1329	WP_026916468.1	exodeoxyribonuclease VII large subunit	-
B4U42_RS06775	1243143..1244405	-	1263	WP_005712610.1	serine hydroxymethyltransferase	-
B4U42_RS06780	1244759..1245769	-	1011	WP_005712612.1	ornithine carbamoyltransferase	-
B4U42_RS06785	1245916..1246194	+	279	WP_005712614.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B4U42_RS06790	1246205..1246477	+	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
B4U42_RS06795	1246541..1247761	+	1221	WP_005712618.1	ATP-dependent RNA helicase RhlB	-
B4U42_RS06800	1247771..1248079	+	309	Protein_1261	DUF853 family protein	-
B4U42_RS06805	1248166..1249152	-	987	WP_005714908.1	IS110 family transposase	-
B4U42_RS06810	1249359..1250573	-	1215	WP_021114868.1	ATP-binding protein	-
B4U42_RS06815	1250780..1251319	-	540	WP_005714441.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10326.71 Da
• Isoelectric Point: 10.0505

>T76878 WP_005712614.1 NZ_CP021644:1245916-1246194 [Glaesserella parasuis 29755]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKFQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T76878 NZ_CP021644:1245916-1246194 [Glaesserella parasuis 29755]
ATGATTATTTCATTTAAGCATAAAGGATTGAAATTATTTTTTGAAACAGGATCAACCGCAGGTATTCAAGCAAATCACGC
TAGTAAACTTGCTTTTCAATTAGCCACTCTAAACCAAGCAAAAAGTGCTTTAGATATGAATGCACCTAGCTGGAATTTAC
ATCAATTACAAGGTAATTTAGCTGGGCATTGGTCTATCAAGGTAAGTGGAAATTGGCGTTTAACTTTTAAATTTCAAAAT
GGTCACGCTGAAGTCGTGGACTATCAAGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT76878 WP_005712616.1 NZ_CP021644:1246205-1246477 [Glaesserella parasuis 29755]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT76878 NZ_CP021644:1246205-1246477 [Glaesserella parasuis 29755]
ATGAAAATGTACAATCCAGCTCACCCAGGGCTTATTTTAAGAGAATATATTGAAGGATATAATGTCACAGAAGTGGCTAA
ACGTTTAGGTGTCACTCGTGTCACGCTCTCTCGTATTTTAAATGGCAAAGCCGCAATTTCACCTGAAATGGCTCTTAGAT
TAAGCCAATTATTAAAAACTAGCCCAGAATTATGGCTTGGTTTACAATCCCAATACGATCTTTGGCAGCTTGAACAACGC
CCAAGTTTTAATATTCAGCCGTTATTTGCATAG

Structures

Toxin

• AlphaFold DB: A0A143CE91