Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 2078443..2078984 Replicon chromosome
Accession NZ_CP021641
Organism Alcaligenes faecalis strain JQ135

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag AFA_RS09675 Protein ID WP_094196740.1
Coordinates 2078443..2078769 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag AFA_RS09680 Protein ID WP_086060438.1
Coordinates 2078766..2078984 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFA_RS09655 2073512..2074138 + 627 WP_094196736.1 tyrosine-type recombinase/integrase -
AFA_RS09660 2074277..2075962 + 1686 WP_159063923.1 hypothetical protein -
AFA_RS09665 2076241..2077944 - 1704 WP_094196738.1 serine/threonine protein kinase -
AFA_RS09675 2078443..2078769 - 327 WP_094196740.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AFA_RS09680 2078766..2078984 - 219 WP_086060438.1 antitoxin MazE family protein Antitoxin
AFA_RS09685 2079147..2079518 - 372 WP_094196741.1 hypothetical protein -
AFA_RS09690 2079671..2080477 + 807 WP_094196742.1 SprT-like domain-containing protein -
AFA_RS09695 2080549..2081058 + 510 WP_094196743.1 PH domain-containing protein -
AFA_RS09700 2081080..2081838 + 759 WP_094196744.1 DsbC family protein -
AFA_RS18775 2081851..2082450 + 600 WP_159063924.1 hypothetical protein -
AFA_RS09710 2082459..2083352 + 894 WP_094196746.1 hypothetical protein -
AFA_RS09715 2083455..2083775 + 321 WP_094196747.1 H-NS histone family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2070442..2111247 40805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-105)

Antitoxin

Antitoxin MazE-like

(6-70)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11629.69 Da        Isoelectric Point: 9.1191

>T76869 WP_094196740.1 NZ_CP021641:c2078769-2078443 [Alcaligenes faecalis]
MRRGDLVTVAMQGDFGKPRPALVIQADLFAAHSSVTVLPVTSTIVNAPLLRISIQPDLENGLQKPSQIMIDKALTVKRDK
IGQAFGYVGADTLVEIERCLAVFLGIAK

Download         Length: 327 bp

>T76869 NZ_CP021641:c2078769-2078443 [Alcaligenes faecalis]
ATGAGGCGTGGGGATCTAGTTACTGTTGCAATGCAAGGGGATTTTGGAAAGCCTCGGCCTGCATTAGTGATCCAAGCGGA
TTTGTTCGCTGCCCATTCCTCGGTCACAGTGCTCCCAGTAACCAGCACAATTGTGAATGCGCCCTTATTGCGCATTAGCA
TTCAGCCAGACCTAGAAAATGGCCTACAAAAACCATCACAAATCATGATCGATAAGGCATTGACCGTTAAACGAGATAAA
ATTGGCCAGGCCTTTGGGTATGTTGGCGCTGATACACTGGTGGAAATTGAACGGTGCCTTGCTGTTTTCTTGGGAATCGC
TAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8259.27 Da        Isoelectric Point: 6.4577

>AT76869 WP_086060438.1 NZ_CP021641:c2078984-2078766 [Alcaligenes faecalis]
MANVNARVQKHRDSLRQAGLRPVQLWVPDTRRPDFAAECRRQALRVAKADKADVDTEHFMDEALADLDGWTE

Download         Length: 219 bp

>AT76869 NZ_CP021641:c2078984-2078766 [Alcaligenes faecalis]
ATGGCAAATGTAAACGCGAGAGTGCAGAAGCACCGAGATTCGCTCCGGCAAGCAGGATTGCGCCCAGTGCAGCTTTGGGT
GCCTGATACACGTCGGCCTGATTTTGCGGCGGAATGCCGTCGTCAGGCGCTGCGGGTAGCCAAGGCAGATAAGGCTGACG
TGGATACAGAACATTTTATGGATGAGGCTCTGGCTGACCTGGATGGTTGGACAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References