Detailed information of TA system TA076865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 1464694..1465260
• Replicon: chromosome
• Accession: NZ_CP021558
• Organism: Bifidobacterium breve strain 215W447a

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W6EX94
• Locus tag: BB215W447A_RS06925
• Protein ID: WP_003831807.1
• Coordinates: 1464967..1465260 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BB215W447A_RS06920
• Protein ID: WP_025221722.1
• Coordinates: 1464694..1464963 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB215W447A_RS06895	1460443..1461138	-	696	WP_003829410.1	response regulator transcription factor	-
BB215W447A_RS06900	1461264..1461779	+	516	WP_013140733.1	hypothetical protein	-
BB215W447A_RS06905	1461776..1462666	+	891	WP_003831809.1	hypothetical protein	-
BB215W447A_RS06910	1462663..1463343	+	681	WP_003829413.1	ABC transporter ATP-binding protein	-
BB215W447A_RS06915	1463340..1464509	+	1170	WP_106641557.1	ABC transporter permease	-
BB215W447A_RS06920	1464694..1464963	+	270	WP_025221722.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BB215W447A_RS06925	1464967..1465260	+	294	WP_003831807.1	type II toxin-antitoxin system YafQ family toxin	Toxin
BB215W447A_RS06935	1465929..1466480	+	552	WP_065454381.1	RNA polymerase sigma factor	-
BB215W447A_RS06940	1466514..1467341	+	828	WP_106641558.1	hypothetical protein	-
BB215W447A_RS06945	1467473..1468099	+	627	WP_106641559.1	hypothetical protein	-
BB215W447A_RS06950	1468096..1468878	+	783	WP_106641560.1	murein hydrolase transporter LrgB	-
BB215W447A_RS06955	1468932..1469807	+	876	WP_106641799.1	ABC transporter ATP-binding protein	-
BB215W447A_RS06960	1469862..1470260	+	399	WP_106641561.1	superoxide dismutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1458598..1476689	18091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-95)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-88)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11218.98 Da
• Isoelectric Point: 9.7881

>T76865 WP_003831807.1 NZ_CP021558:1464967-1465260 [Bifidobacterium breve]
MLKPDYTAAFQRDIKKLKRKHTDLRPLKEVIRLVLEDTADSKEVLRRRHRAHTLTGDLNGVLECHIGNAGDWLLLWIRDD
GTAMFMRTGSHDELLGK

Nucleotide

• Length: 294 bp

>T76865 NZ_CP021558:1464967-1465260 [Bifidobacterium breve]
ATGCTCAAACCGGATTACACAGCGGCATTCCAGCGAGATATCAAGAAACTCAAACGCAAGCACACTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTTCTTGAGGACACGGCGGACTCCAAGGAAGTATTGAGGCGTAGGCATCGCGCCCACACAC
TTACCGGTGATTTAAACGGCGTACTGGAATGCCACATCGGCAATGCCGGCGACTGGCTGCTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAACTGTTGGGCAAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9859.22 Da
• Isoelectric Point: 6.3186

>AT76865 WP_025221722.1 NZ_CP021558:1464694-1464963 [Bifidobacterium breve]
VSMASVTVRVDADTKQKAANIAEDLGLDLSSVTRAFYKQIVRERRIPLNLSYTTVPPETMKALDEAKNRLADGKPRFNSA
DEMFDSLGI

Nucleotide

• Length: 270 bp

>AT76865 NZ_CP021558:1464694-1464963 [Bifidobacterium breve]
GTGAGCATGGCATCCGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAAACATAGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGCGCCTTCTACAAGCAGATTGTCCGGGAGAGGCGCATTCCTCTAAACCTTTCCTATACGA
CCGTGCCGCCTGAAACCATGAAGGCCCTTGACGAGGCGAAAAACAGGCTCGCCGACGGCAAACCTCGTTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGGAATCTGA

Structures

Toxin

• AlphaFold DB: W6EX94