Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1004063..1004618 Replicon chromosome
Accession NZ_CP021558
Organism Bifidobacterium breve strain 215W447a

Toxin (Protein)


Gene name mazF Uniprot ID B7GSS5
Locus tag BB215W447A_RS04360 Protein ID WP_003833460.1
Coordinates 1004063..1004386 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag BB215W447A_RS04365 Protein ID WP_003829112.1
Coordinates 1004373..1004618 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB215W447A_RS04325 999106..999394 - 289 Protein_805 IS256 family transposase -
BB215W447A_RS04335 999865..1001130 - 1266 WP_106641369.1 Fic family protein -
BB215W447A_RS04345 1001665..1002030 - 366 WP_106641370.1 YccF domain-containing protein -
BB215W447A_RS04350 1002268..1003137 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
BB215W447A_RS04355 1003232..1003846 + 615 WP_106641371.1 cupin domain-containing protein -
BB215W447A_RS04360 1004063..1004386 - 324 WP_003833460.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BB215W447A_RS04365 1004373..1004618 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BB215W447A_RS04370 1004790..1006046 + 1257 WP_015438708.1 HipA domain-containing protein -
BB215W447A_RS04375 1006312..1006490 + 179 Protein_813 XRE family transcriptional regulator -
BB215W447A_RS04380 1006674..1007945 - 1272 WP_052790209.1 MFS transporter -
BB215W447A_RS04385 1008102..1008764 - 663 WP_106641372.1 TetR/AcrR family transcriptional regulator -
BB215W447A_RS12240 1009226..1009411 + 186 WP_003808830.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-106)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11829.64 Da        Isoelectric Point: 6.2932

>T76863 WP_003833460.1 NZ_CP021558:c1004386-1004063 [Bifidobacterium breve]
MKRGEIRTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T76863 NZ_CP021558:c1004386-1004063 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGTGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT76863 WP_003829112.1 NZ_CP021558:c1004618-1004373 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT76863 NZ_CP021558:c1004618-1004373 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0SL31

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References