Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1060779..1061415 Replicon chromosome
Accession NZ_CP021555
Organism Bifidobacterium breve strain 082W48

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag BB082W48_RS04570 Protein ID WP_003829234.1
Coordinates 1060779..1061135 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag BB082W48_RS04575 Protein ID WP_012577921.1
Coordinates 1061122..1061415 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB082W48_RS04540 1055969..1056880 + 912 WP_106622278.1 ABC transporter ATP-binding protein -
BB082W48_RS04545 1057077..1057703 + 627 WP_003829226.1 30S ribosomal protein S4 -
BB082W48_RS04550 1058040..1058822 - 783 WP_003829227.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
BB082W48_RS04555 1058965..1059450 - 486 WP_003829229.1 cytidine deaminase -
BB082W48_RS04560 1059539..1059895 + 357 WP_052788397.1 SdpI family protein -
BB082W48_RS04565 1060000..1060602 + 603 WP_106622279.1 transglutaminase family protein -
BB082W48_RS04570 1060779..1061135 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BB082W48_RS04575 1061122..1061415 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BB082W48_RS04580 1061566..1061730 - 165 Protein_857 IS30 family transposase -
BB082W48_RS04585 1061830..1062504 + 675 WP_015438748.1 histidine phosphatase family protein -
BB082W48_RS04590 1062587..1062970 + 384 WP_016463075.1 DUF948 domain-containing protein -
BB082W48_RS04595 1062970..1063206 + 237 WP_003829239.1 hypothetical protein -
BB082W48_RS04600 1063302..1065980 + 2679 WP_003829240.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T76857 WP_003829234.1 NZ_CP021555:c1061135-1060779 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T76857 NZ_CP021555:c1061135-1060779 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT76857 WP_012577921.1 NZ_CP021555:c1061415-1061122 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT76857 NZ_CP021555:c1061415-1061122 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References