Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2325291..2325793 Replicon chromosome
Accession NZ_CP021535
Organism Escherichia coli strain AR_0119

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM366_RS11960 Protein ID WP_000767829.1
Coordinates 2325539..2325793 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM366_RS11955 Protein ID WP_001259255.1
Coordinates 2325291..2325542 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM366_RS11925 2320469..2321536 - 1068 WP_000080109.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AM366_RS11930 2321536..2322606 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
AM366_RS11935 2322603..2323907 - 1305 WP_001345878.1 histidinol dehydrogenase -
AM366_RS11940 2323913..2324812 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
AM366_RS11950 2324958..2325008 - 51 WP_001364200.1 his operon leader peptide -
AM366_RS11955 2325291..2325542 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM366_RS11960 2325539..2325793 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM366_RS11965 2325876..2326700 + 825 WP_087757962.1 SDR family oxidoreductase -
AM366_RS11970 2326746..2327675 + 930 WP_000803351.1 LysR family transcriptional regulator -
AM366_RS29095 2327890..2327952 + 63 WP_010723108.1 membrane protein YoeI -
AM366_RS11975 2327942..2329300 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM366_RS11980 2329479..2330537 + 1059 WP_000492324.1 transport protein YeeE -
AM366_RS11985 2330551..2330778 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T76771 WP_000767829.1 NZ_CP021535:2325539-2325793 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T76771 NZ_CP021535:2325539-2325793 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT76771 WP_001259255.1 NZ_CP021535:2325291-2325542 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT76771 NZ_CP021535:2325291-2325542 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References