Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1889948..1890450 Replicon chromosome
Accession NZ_CP021454
Organism Escherichia coli strain H105

Toxin (Protein)


Gene name yoeB Uniprot ID E2QNP2
Locus tag CCL28_RS09385 Protein ID WP_000767819.1
Coordinates 1890196..1890450 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID E2QNP3
Locus tag CCL28_RS09380 Protein ID WP_001259253.1
Coordinates 1889948..1890199 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCL28_RS09350 1885126..1886193 - 1068 WP_000080057.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CCL28_RS09355 1886193..1887263 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
CCL28_RS09360 1887260..1888564 - 1305 WP_001296211.1 histidinol dehydrogenase -
CCL28_RS09365 1888570..1889469 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CCL28_RS09375 1889615..1889665 - 51 WP_001364200.1 his operon leader peptide -
CCL28_RS09380 1889948..1890199 + 252 WP_001259253.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CCL28_RS09385 1890196..1890450 + 255 WP_000767819.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CCL28_RS09390 1890533..1891357 + 825 WP_000754737.1 SDR family oxidoreductase -
CCL28_RS09395 1891403..1892332 + 930 WP_000803366.1 LysR family transcriptional regulator -
CCL28_RS26755 1892547..1892609 + 63 WP_010723108.1 membrane protein YoeI -
CCL28_RS09400 1892599..1893957 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -
CCL28_RS09410 1894174..1894632 + 459 WP_001531805.1 IS200/IS605-like element IS200C family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1894174..1894632 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10110.53 Da        Isoelectric Point: 7.2749

>T76680 WP_000767819.1 NZ_CP021454:1890196-1890450 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T76680 NZ_CP021454:1890196-1890450 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9365.62 Da        Isoelectric Point: 4.6127

>AT76680 WP_001259253.1 NZ_CP021454:1889948-1890199 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Download         Length: 252 bp

>AT76680 NZ_CP021454:1889948-1890199 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E2QNP2

Antitoxin

Source ID Structure
AlphaFold DB E2QNP3

References