Detailed information of TA system TA076659

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2733912..2734121
• Replicon: chromosome
• Accession: NZ_CP021447
• Organism: Clostridioides difficile strain DSM 104451

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: CDIF104451_RS12915
• Protein ID: WP_004454815.1
• Coordinates: 2733963..2734121 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2733912..2734046 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF104451_RS12900 (2729803)	2729803..2731431	+	1629	WP_004454813.1	aspartate 4-decarboxylase	-
CDIF104451_RS12905 (2731552)	2731552..2732547	+	996	WP_003431031.1	asparaginase	-
CDIF104451_RS12910 (2732736)	2732736..2732984	-	249	WP_231298879.1	BlaI/MecI/CopY family transcriptional regulator	-
- (2733912)	2733912..2734046	+	135	NuclAT_2	-	Antitoxin
- (2733912)	2733912..2734046	+	135	NuclAT_2	-	Antitoxin
- (2733912)	2733912..2734046	+	135	NuclAT_4	-	Antitoxin
CDIF104451_RS12915 (2733963)	2733963..2734121	-	159	WP_004454815.1	hypothetical protein	Toxin
CDIF104451_RS12920 (2734480)	2734480..2735850	-	1371	WP_021360826.1	cell wall-binding protein Cwp29	-
CDIF104451_RS12925 (2736286)	2736286..2736372	+	87	WP_231298880.1	hypothetical protein	-
CDIF104451_RS12930 (2736416)	2736416..2736715	-	300	WP_225722727.1	hypothetical protein	-
CDIF104451_RS12935 (2736902)	2736902..2737174	+	273	WP_004454820.1	tyrosine-type recombinase/integrase	-
CDIF104451_RS12940 (2737548)	2737548..2737628	-	81	Protein_2473	VOC family protein	-
CDIF104451_RS12945 (2737668)	2737668..2738336	-	669	WP_009897711.1	VanZ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T76659 WP_004454815.1 NZ_CP021447:c2734121-2733963 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T76659 NZ_CP021447:c2734121-2733963 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT76659 NZ_CP021447:2733912-2734046 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin