Detailed information of TA system TA076577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 1225644..1226210
• Replicon: chromosome
• Accession: NZ_CP021387
• Organism: Bifidobacterium breve strain NRBB09

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: NRBB09_RS05605
• Protein ID: WP_106629487.1
• Coordinates: 1225917..1226210 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I3BGP0
• Locus tag: NRBB09_RS05600
• Protein ID: WP_007056990.1
• Coordinates: 1225644..1225913 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NRBB09_RS05570	1221370..1222383	-	1014	WP_198552122.1	VanZ family protein	-
NRBB09_RS05575	1222530..1223354	+	825	WP_003829401.1	aminotransferase class V-fold PLP-dependent enzyme	-
NRBB09_RS05580	1223540..1223674	+	135	WP_077320901.1	DUF2316 family protein	-
NRBB09_RS05585	1223715..1224248	-	534	WP_014483715.1	GNAT family N-acetyltransferase	-
NRBB09_RS10140	1224504..1224953	+	450	WP_157824053.1	IS3 family transposase	-
NRBB09_RS05595	1224962..1225459	+	498	Protein_1046	FtsX-like permease family protein	-
NRBB09_RS05600	1225644..1225913	+	270	WP_007056990.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
NRBB09_RS05605	1225917..1226210	+	294	WP_106629487.1	type II toxin-antitoxin system YafQ family toxin	Toxin
NRBB09_RS05610	1226267..1226501	+	235	Protein_1049	hypothetical protein	-
NRBB09_RS05615	1226503..1227400	+	898	Protein_1050	MFS transporter	-
NRBB09_RS05620	1227724..1229304	+	1581	WP_025262999.1	amino acid permease	-
NRBB09_RS05625	1229546..1229785	-	240	WP_003829454.1	hypothetical protein	-
NRBB09_RS05630	1230082..1230435	+	354	WP_014483707.1	DUF488 domain-containing protein	-
NRBB09_RS10245	1230501..1230929	-	429	WP_015438848.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-94)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-87)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11207.96 Da
• Isoelectric Point: 10.1999

>T76577 WP_106629487.1 NZ_CP021387:1225917-1226210 [Bifidobacterium breve]
MLKPDYTAAFQRDIKKLKRRHADLRPLKEVIRLVLEDTADSKEALRRRHRAHTLTGDLNGVLECRIGNAGDWLLLWIRDD
GTAMFMRTGSHDELLGK

Nucleotide

• Length: 294 bp

>T76577 NZ_CP021387:1225917-1226210 [Bifidobacterium breve]
ATGCTCAAACCGGATTACACGGCGGCATTCCAGCGAGATATCAAGAAGCTCAAACGCAGGCACGCTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTGCTTGAGGACACGGCGGACTCCAAGGAAGCATTGAGGCGTAGGCATCGCGCCCACACAC
TTACCGGTGATCTAAACGGCGTACTGGAATGCCGCATCGGCAATGCCGGCGACTGGCTGCTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAACTGTTGGGCAAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9844.16 Da
• Isoelectric Point: 4.5742

>AT76577 WP_007056990.1 NZ_CP021387:1225644-1225913 [Bifidobacterium breve]
VSMASVTVRVDADTKQKAANIAEDLGLDLSSVTRAFYKQIVREKRIPLNLSYTIVPPETMEALDEAKNRLADGQPRFNSA
DEMFDSLGI

Nucleotide

• Length: 270 bp

>AT76577 NZ_CP021387:1225644-1225913 [Bifidobacterium breve]
GTGAGCATGGCATCCGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAAACATAGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGCGCCTTCTACAAGCAGATTGTCCGGGAGAAGCGCATTCCCCTAAACCTTTCCTATACGA
TCGTGCCGCCTGAAACCATGGAGGCCCTTGACGAGGCGAAGAACAGGCTCGCCGACGGCCAACCTCGCTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGGAATCTGA

Structures

Antitoxin

• AlphaFold DB: I3BGP0