Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1013416..1014052 Replicon chromosome
Accession NZ_CP021387
Organism Bifidobacterium breve strain NRBB09

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag NRBB09_RS04500 Protein ID WP_003829234.1
Coordinates 1013416..1013772 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag NRBB09_RS04505 Protein ID WP_012577921.1
Coordinates 1013759..1014052 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRBB09_RS04470 1008609..1009520 + 912 WP_106629428.1 ABC transporter ATP-binding protein -
NRBB09_RS04475 1009717..1010343 + 627 WP_003829226.1 30S ribosomal protein S4 -
NRBB09_RS04480 1010680..1011462 - 783 WP_106629429.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
NRBB09_RS04485 1011603..1012088 - 486 WP_003829229.1 cytidine deaminase -
NRBB09_RS04490 1012176..1012532 + 357 WP_052788397.1 SdpI family protein -
NRBB09_RS04495 1012637..1013239 + 603 WP_106629430.1 transglutaminase family protein -
NRBB09_RS04500 1013416..1013772 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRBB09_RS04505 1013759..1014052 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NRBB09_RS04510 1014203..1014367 - 165 Protein_837 IS30 family transposase -
NRBB09_RS04515 1014467..1015141 + 675 WP_015438748.1 histidine phosphatase family protein -
NRBB09_RS04520 1015224..1015607 + 384 WP_106629431.1 DUF948 domain-containing protein -
NRBB09_RS04525 1015607..1015843 + 237 WP_003829239.1 hypothetical protein -
NRBB09_RS04530 1015939..1018617 + 2679 WP_106629432.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T76576 WP_003829234.1 NZ_CP021387:c1013772-1013416 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T76576 NZ_CP021387:c1013772-1013416 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT76576 WP_012577921.1 NZ_CP021387:c1014052-1013759 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT76576 NZ_CP021387:c1014052-1013759 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References