Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 967144..967699 Replicon chromosome
Accession NZ_CP021384
Organism Bifidobacterium breve strain NRBB01

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag NRBB01_RS04265 Protein ID WP_003829109.1
Coordinates 967144..967467 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag NRBB01_RS04270 Protein ID WP_003829112.1
Coordinates 967454..967699 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRBB01_RS04230 962181..962469 - 289 Protein_780 IS256 family transposase -
NRBB01_RS04240 962940..964205 - 1266 WP_106620598.1 Fic family protein -
NRBB01_RS04250 964747..965112 - 366 WP_032738175.1 YccF domain-containing protein -
NRBB01_RS04255 965350..966219 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
NRBB01_RS04260 966313..966927 + 615 WP_003829107.1 cupin domain-containing protein -
NRBB01_RS04265 967144..967467 - 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRBB01_RS04270 967454..967699 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NRBB01_RS04275 967873..969129 + 1257 WP_003829116.1 HipA domain-containing protein -
NRBB01_RS04280 969394..969585 + 192 WP_003829118.1 hypothetical protein -
NRBB01_RS04285 969769..971040 - 1272 WP_003829121.1 MFS transporter -
NRBB01_RS04290 971197..971859 - 663 WP_025262926.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 956913..1005197 48284


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T76568 WP_003829109.1 NZ_CP021384:c967467-967144 [Bifidobacterium breve]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T76568 NZ_CP021384:c967467-967144 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT76568 WP_003829112.1 NZ_CP021384:c967699-967454 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT76568 NZ_CP021384:c967699-967454 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References