Detailed information of TA system TA076358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2654666..2656100
• Replicon: chromosome
• Accession: NZ_CP021264
• Organism: Legionella pneumophila subsp. fraseri strain D-5387

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: A0A2S6EXC5
• Locus tag: CA928_RS11855
• Protein ID: WP_032830191.1
• Coordinates: 2654880..2656100 (+)
• Length: 407 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: CA928_RS11850
• Protein ID: WP_027227407.1
• Coordinates: 2654666..2654893 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA928_RS11825	2650461..2651327	-	867	WP_027227403.1	transposase	-
CA928_RS11830	2651389..2651577	-	189	WP_071974648.1	helix-turn-helix domain-containing protein	-
CA928_RS11835	2651766..2652554	+	789	WP_027227404.1	hypothetical protein	-
CA928_RS11840	2652619..2653029	-	411	Protein_2293	transposase family protein	-
CA928_RS11845	2653148..2654170	-	1023	WP_027227406.1	hypothetical protein	-
CA928_RS11850	2654666..2654893	+	228	WP_027227407.1	helix-turn-helix transcriptional regulator	Antitoxin
CA928_RS11855	2654880..2656100	+	1221	WP_032830191.1	type II toxin-antitoxin system HipA family toxin	Toxin
CA928_RS11860	2656135..2656491	-	357	WP_027227408.1	preprotein translocase	-
CA928_RS11865	2656531..2657436	-	906	WP_027227409.1	LysR family transcriptional regulator	-
CA928_RS11870	2657527..2658387	+	861	WP_027227410.1	DUF2608 domain-containing protein	-
CA928_RS11875	2658739..2659003	-	265	Protein_2300	hypothetical protein	-
CA928_RS11880	2659051..2659362	-	312	WP_027227411.1	cupin	-
CA928_RS11885	2659378..2659626	-	249	WP_031301595.1	hypothetical protein	-
CA928_RS11890	2659933..2660406	+	474	WP_027227413.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (147-380)

Antitoxin

• Transcription regulator, HipB-like (7-63)

Sequences

Toxin

Protein

• Length: 407 a.a.
• Molecular weight: 45374.54 Da
• Isoelectric Point: 8.3174

>T76358 WP_032830191.1 NZ_CP021264:2654880-2656100 [Legionella pneumophila subsp. fraseri]
MKNHKLDIYLNQTLAGQLRIDTHGEMSFVYDEGYLENNKNLPLSHSLPLQQAPYSTKQCRPFFSGLLPEAHMRTAIARQL
GISEKNDFALLAAIGGECAGAVMILPQDTSITQLKPDYKIIKKQDVLEILQTMHQRPMIVGEDGIRLSLAGAQDKLPVAI
IEGNLAIPMNGAPSTHILKPINRDFPSLIENESFCLNLAQKIGLNAVDASIHYADGTPYLLVKRYDRVETKQGIQRLHQE
DFCQAMGISPEMKYQREGGPQISECFGLIREISSLPVLDIKELLQGILFNVIIGNNDAHSKNFSLLYHGSKVRLAPFYDL
ISTVYYPNLASKMAMKVGSKYDFDGLFPRHFIQMAEEARLSGALVRKEVLNLISKIQKNITASPFTSIILQRADKLFQKF
QKASTG

Nucleotide

• Length: 1221 bp

>T76358 NZ_CP021264:2654880-2656100 [Legionella pneumophila subsp. fraseri]
ATGAAAAATCATAAACTTGATATCTATCTTAACCAAACACTAGCAGGCCAATTACGTATCGATACGCATGGCGAGATGTC
TTTTGTGTATGATGAAGGCTACCTGGAAAACAATAAAAATCTCCCGTTGTCACACTCCCTTCCCCTTCAACAAGCCCCTT
ACTCAACGAAGCAATGCCGTCCGTTTTTTAGCGGACTATTGCCTGAAGCCCATATGCGTACTGCTATTGCCCGTCAATTG
GGTATCAGTGAAAAAAATGATTTTGCCTTATTAGCAGCTATTGGTGGTGAGTGTGCCGGAGCTGTGATGATATTACCTCA
AGATACATCCATAACTCAGCTAAAGCCAGACTATAAAATTATCAAAAAACAGGATGTATTAGAAATCCTGCAAACCATGC
ATCAAAGACCCATGATAGTCGGTGAGGATGGAATTCGTTTATCCCTTGCTGGAGCTCAGGATAAACTTCCAGTTGCCATT
ATTGAAGGAAATCTCGCAATACCAATGAACGGTGCACCGAGTACTCATATTTTAAAACCAATTAATCGAGACTTTCCCTC
CTTAATTGAAAATGAATCTTTCTGCCTTAATCTGGCACAAAAAATTGGATTAAATGCTGTGGACGCATCAATCCATTATG
CTGATGGTACCCCTTATCTGTTGGTTAAACGTTATGATCGGGTTGAGACAAAGCAAGGAATCCAACGTCTTCATCAAGAA
GATTTTTGTCAGGCAATGGGCATTAGCCCTGAAATGAAATATCAGCGAGAAGGCGGCCCCCAAATTAGTGAGTGCTTTGG
TTTGATAAGAGAGATATCAAGCCTCCCTGTCCTTGACATAAAAGAATTATTACAAGGGATTCTGTTTAATGTCATTATTG
GCAACAATGATGCACATAGCAAAAACTTCTCTTTGCTCTATCATGGTTCAAAAGTTCGTTTAGCACCTTTCTACGATCTG
ATTTCAACCGTATATTATCCTAATCTTGCCAGTAAAATGGCGATGAAAGTTGGAAGTAAATATGATTTTGATGGCCTATT
TCCACGCCACTTTATACAAATGGCAGAGGAGGCTCGTTTATCGGGTGCCTTAGTGCGTAAAGAAGTCTTGAATCTAATTA
GCAAAATTCAAAAGAACATAACCGCCAGCCCCTTTACCAGCATCATTTTGCAACGTGCTGACAAATTATTTCAAAAGTTC
CAGAAGGCTTCAACAGGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8467.85 Da
• Isoelectric Point: 9.1200

>AT76358 WP_027227407.1 NZ_CP021264:2654666-2654893 [Legionella pneumophila subsp. fraseri]
MIQSPENIGRYIRKVRKELNVTQKDLALTAGTGLRFIIDLENGKPTCQIGKMLQVLQVLGIQLNLSHTDFENEKS

Nucleotide

• Length: 228 bp

>AT76358 NZ_CP021264:2654666-2654893 [Legionella pneumophila subsp. fraseri]
ATGATTCAATCTCCAGAAAATATAGGACGTTATATTCGCAAAGTTCGTAAAGAACTTAATGTTACCCAAAAAGATTTGGC
TTTAACTGCTGGAACTGGATTGCGTTTTATTATCGATTTGGAAAATGGAAAGCCCACTTGCCAAATTGGAAAAATGCTGC
AAGTACTACAAGTACTTGGGATTCAACTCAACTTATCTCATACTGATTTCGAAAATGAAAAATCATAA

Structures

Toxin

• AlphaFold DB: A0A2S6EXC5