Detailed information of TA system TA076299

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3792937..3793772
• Replicon: chromosome
• Accession: NZ_CP021207
• Organism: Escherichia coli strain strain Z247

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CA270_RS19495
• Protein ID: WP_001094425.1
• Coordinates: 3793395..3793772 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: CA270_RS19490
• Protein ID: WP_001285575.1
• Coordinates: 3792937..3793305 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA270_RS19455	3788825..3789505	+	681	WP_001282912.1	WYL domain-containing protein	-
CA270_RS19460	3789656..3790333	+	678	WP_021520461.1	hypothetical protein	-
CA270_RS19465	3790339..3790572	+	234	WP_001278287.1	DUF905 family protein	-
CA270_RS19470	3790662..3791480	+	819	WP_001175163.1	DUF945 domain-containing protein	-
CA270_RS19475	3791572..3792057	+	486	WP_021524627.1	antirestriction protein	-
CA270_RS19480	3792073..3792549	+	477	WP_001186771.1	RadC family protein	-
CA270_RS19485	3792636..3792857	+	222	WP_000692350.1	DUF987 domain-containing protein	-
CA270_RS19490	3792937..3793305	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CA270_RS19495	3793395..3793772	+	378	WP_001094425.1	TA system toxin CbtA family protein	Toxin
CA270_RS19500	3793769..3794257	+	489	WP_000761683.1	hypothetical protein	-
CA270_RS19505	3794269..3794466	+	198	WP_000839272.1	DUF957 domain-containing protein	-
CA270_RS28775	3794545..3794703	+	159	WP_001467148.1	hypothetical protein	-
CA270_RS19515	3795247..3795418	+	172	Protein_3583	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14058.97 Da
• Isoelectric Point: 7.3523

>T76299 WP_001094425.1 NZ_CP021207:3793395-3793772 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACSRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T76299 NZ_CP021207:3793395-3793772 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTAGTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT76299 WP_001285575.1 NZ_CP021207:3792937-3793305 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT76299 NZ_CP021207:3792937-3793305 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A454ABF5