Detailed information of TA system TA076293

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2178161..2178993
• Replicon: chromosome
• Accession: NZ_CP021207
• Organism: Escherichia coli strain strain Z247

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: CA270_RS11265
• Protein ID: WP_000854753.1
• Coordinates: 2178619..2178993 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: CA270_RS11260
• Protein ID: WP_001278232.1
• Coordinates: 2178161..2178529 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA270_RS11220	2174997..2175452	+	456	WP_000581504.1	hypothetical protein	-
CA270_RS11225	2175531..2175764	+	234	WP_001119729.1	DUF905 family protein	-
CA270_RS11235	2175864..2176682	+	819	WP_086353160.1	DUF945 domain-containing protein	-
CA270_RS11240	2176737..2177222	+	486	WP_032286264.1	antirestriction protein	-
CA270_RS11245	2177238..2177714	+	477	WP_001186727.1	RadC family protein	-
CA270_RS11250	2177777..2177998	+	222	WP_021532904.1	DUF987 domain-containing protein	-
CA270_RS11260	2178161..2178529	+	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
CA270_RS11265	2178619..2178993	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
CA270_RS11270	2178990..2179478	+	489	WP_000777541.1	hypothetical protein	-
CA270_RS11275	2179490..2179687	+	198	WP_000839281.1	DUF957 domain-containing protein	-
CA270_RS11280	2179784..2180071	+	288	Protein_2077	DUF4942 domain-containing protein	-
CA270_RS11285	2180143..2181147	+	1005	WP_001179707.1	IS110-like element ISSfl8 family transposase	-
CA270_RS11290	2181429..2181710	+	282	Protein_2079	DUF4942 domain-containing protein	-
CA270_RS11295	2182053..2182223	+	171	Protein_2080	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2180143..2181147	1004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T76293 WP_000854753.1 NZ_CP021207:2178619-2178993 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T76293 NZ_CP021207:2178619-2178993 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCCGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT76293 WP_001278232.1 NZ_CP021207:2178161-2178529 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT76293 NZ_CP021207:2178161-2178529 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: E3PJ72