Detailed information of TA system TA076291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1150550..1151052
• Replicon: chromosome
• Accession: NZ_CP021207
• Organism: Escherichia coli strain strain Z247

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: CA270_RS06270
• Protein ID: WP_000767819.1
• Coordinates: 1150550..1150804 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: CA270_RS06275
• Protein ID: WP_001259253.1
• Coordinates: 1150801..1151052 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA270_RS06245	1146368..1146826	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
CA270_RS06255	1147043..1148401	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
CA270_RS28315	1148391..1148453	-	63	WP_010723108.1	membrane protein YoeI	-
CA270_RS06260	1148668..1149597	-	930	WP_000803366.1	LysR family transcriptional regulator	-
CA270_RS06265	1149643..1150467	-	825	WP_000754737.1	SDR family oxidoreductase	-
CA270_RS06270	1150550..1150804	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
CA270_RS06275	1150801..1151052	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
CA270_RS06280	1151335..1151385	+	51	WP_001364200.1	his operon leader peptide	-
CA270_RS06290	1151531..1152430	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
CA270_RS06295	1152436..1153740	+	1305	WP_001296211.1	histidinol dehydrogenase	-
CA270_RS06300	1153737..1154807	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
CA270_RS06305	1154807..1155874	+	1068	WP_024008731.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1146368..1146826	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T76291 WP_000767819.1 NZ_CP021207:c1150804-1150550 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T76291 NZ_CP021207:c1150804-1150550 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT76291 WP_001259253.1 NZ_CP021207:c1151052-1150801 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT76291 NZ_CP021207:c1151052-1150801 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3