Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3602157..3602793 Replicon chromosome
Accession NZ_CP021123
Organism Bacillus subtilis strain SEM-9

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CAH07_RS18800 Protein ID WP_003156187.1
Coordinates 3602157..3602507 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag CAH07_RS18805 Protein ID WP_003225183.1
Coordinates 3602512..3602793 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAH07_RS18760 3597201..3597800 - 600 WP_003246608.1 phosphoserine phosphatase RsbX -
CAH07_RS18765 3597800..3598588 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
CAH07_RS18770 3598554..3599036 - 483 WP_003234299.1 anti-sigma B factor RsbW -
CAH07_RS18775 3599033..3599362 - 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
CAH07_RS18780 3599424..3600431 - 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
CAH07_RS18785 3600443..3600844 - 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
CAH07_RS18790 3600848..3601213 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
CAH07_RS18795 3601218..3602042 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
CAH07_RS18800 3602157..3602507 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CAH07_RS18805 3602512..3602793 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CAH07_RS18810 3602909..3604078 - 1170 WP_015252766.1 alanine racemase -
CAH07_RS18815 3604193..3605209 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
CAH07_RS18820 3605375..3605740 - 366 WP_014475874.1 holo-ACP synthase -
CAH07_RS18825 3605835..3606434 + 600 WP_129010114.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T76013 WP_003156187.1 NZ_CP021123:c3602507-3602157 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T76013 NZ_CP021123:c3602507-3602157 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT76013 WP_003225183.1 NZ_CP021123:c3602793-3602512 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT76013 NZ_CP021123:c3602793-3602512 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References