Detailed information of TA system TA075714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1867721..1868346
• Replicon: chromosome
• Accession: NZ_CP020933
• Organism: Escherichia coli strain HB-Coli0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B9W17_RS09170
• Protein ID: WP_000911330.1
• Coordinates: 1867948..1868346 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: B9W17_RS09165
• Protein ID: WP_000450524.1
• Coordinates: 1867721..1867948 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9W17_RS09140	1863524..1863994	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
B9W17_RS09145	1863994..1864566	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
B9W17_RS09150	1864712..1865590	+	879	WP_085704045.1	4-hydroxy-tetrahydrodipicolinate synthase	-
B9W17_RS09155	1865607..1866641	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
B9W17_RS09160	1866854..1867567	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
B9W17_RS09165	1867721..1867948	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
B9W17_RS09170	1867948..1868346	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
B9W17_RS09175	1868493..1869356	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
B9W17_RS09180	1869371..1871386	+	2016	WP_063112794.1	tRNA cytosine(34) acetyltransferase TmcA	-
B9W17_RS09185	1871460..1872158	+	699	WP_000679823.1	esterase	-
B9W17_RS09190	1872268..1872468	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T75714 WP_000911330.1 NZ_CP020933:1867948-1868346 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T75714 NZ_CP020933:1867948-1868346 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT75714 WP_000450524.1 NZ_CP020933:1867721-1867948 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT75714 NZ_CP020933:1867721-1867948 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33