Detailed information of TA system TA075705

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 8115..8913
• Replicon: chromosome
• Accession: NZ_CP020933
• Organism: Escherichia coli strain HB-Coli0

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9XMP3
• Locus tag: B9W17_RS00065
• Protein ID: WP_000854735.1
• Coordinates: 8536..8913 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M1EW42
• Locus tag: B9W17_RS00060
• Protein ID: WP_032153712.1
• Coordinates: 8115..8489 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9W17_RS00025	3848..4732	+	885	WP_024166630.1	50S ribosome-binding GTPase	-
B9W17_RS00030	4851..5528	+	678	WP_023155728.1	hypothetical protein	-
B9W17_RS00035	5558..5767	+	210	WP_023155727.1	DUF905 family protein	-
B9W17_RS00040	5858..6676	+	819	WP_001234693.1	DUF945 domain-containing protein	-
B9W17_RS00045	6768..7253	+	486	WP_032153711.1	antirestriction protein	-
B9W17_RS00050	7269..7745	+	477	WP_001186715.1	RadC family protein	-
B9W17_RS00055	7814..8035	+	222	WP_000692345.1	DUF987 domain-containing protein	-
B9W17_RS00060	8115..8489	+	375	WP_032153712.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
B9W17_RS00065	8536..8913	+	378	WP_000854735.1	TA system toxin CbtA family protein	Toxin
B9W17_RS00070	8910..9401	+	492	WP_023155722.1	hypothetical protein	-
B9W17_RS00075	9413..9610	+	198	WP_000839265.1	DUF957 domain-containing protein	-
B9W17_RS00080	9695..10561	+	867	WP_032153714.1	DUF4942 domain-containing protein	-
B9W17_RS00085	10633..10896	+	264	WP_025492088.1	type II toxin-antitoxin system ParD family antitoxin	-
B9W17_RS00090	10893..11219	+	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
B9W17_RS00095	12102..13640	+	1539	WP_001187170.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 8.2904

>T75705 WP_000854735.1 NZ_CP020933:8536-8913 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T75705 NZ_CP020933:8536-8913 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13697.51 Da
• Isoelectric Point: 6.6240

>AT75705 WP_032153712.1 NZ_CP020933:8115-8489 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT75705 NZ_CP020933:8115-8489 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: U9XMP3

Antitoxin

• AlphaFold DB: A0A1M1EW42