Detailed information of TA system TA075689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1874648..1875229
• Replicon: chromosome
• Accession: NZ_CP020918
• Organism: Flavobacterium faecale strain WV33

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: FFWV33_RS08260
• Protein ID: WP_108740459.1
• Coordinates: 1874648..1874947 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FFWV33_RS08265
• Protein ID: WP_108740460.1
• Coordinates: 1874951..1875229 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFWV33_RS08250	1871484..1873262	+	1779	WP_108740457.1	arginine--tRNA ligase	-
FFWV33_RS08255	1873781..1874329	+	549	WP_108740458.1	hypothetical protein	-
FFWV33_RS08260	1874648..1874947	+	300	WP_108740459.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FFWV33_RS08265	1874951..1875229	+	279	WP_108740460.1	putative addiction module antidote protein	Antitoxin
FFWV33_RS08270	1875900..1877804	+	1905	WP_108740461.1	mechanosensitive ion channel family protein	-
FFWV33_RS08275	1877807..1878706	+	900	WP_108740462.1	mechanosensitive ion channel family protein	-
FFWV33_RS08280	1878832..1880193	+	1362	WP_108740463.1	signal recognition particle protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Uncharacterized protein HI1420 (3-88)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11672.62 Da
• Isoelectric Point: 9.9699

>T75689 WP_108740459.1 NZ_CP020918:1874648-1874947 [Flavobacterium faecale]
MYFIEKTSEFDKWLRKLKDLRAKAKILMRVQKLENDEHFGECESVGDGIKELKINYAKGYRVYFKEKDGKIIVLLIGGDK
SSQQSDIEKAKEIWRSLKK

Nucleotide

• Length: 300 bp

>T75689 NZ_CP020918:1874648-1874947 [Flavobacterium faecale]
ATGTACTTTATTGAGAAAACATCAGAATTTGATAAGTGGTTACGAAAACTGAAAGATTTACGTGCCAAAGCAAAAATTTT
AATGAGAGTACAAAAATTGGAAAACGACGAACATTTTGGCGAATGTGAATCAGTTGGTGACGGAATAAAAGAACTTAAAA
TAAATTATGCCAAAGGTTATCGAGTTTACTTCAAAGAAAAAGATGGGAAAATTATAGTCTTATTAATTGGAGGCGACAAA
TCATCTCAACAAAGTGACATTGAAAAGGCAAAAGAAATCTGGAGATCATTAAAAAAATAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9891.34 Da
• Isoelectric Point: 4.6895

>AT75689 WP_108740460.1 NZ_CP020918:1874951-1875229 [Flavobacterium faecale]
METSKFDISDYLDSNEMIAAYLNEVLENGDETDLVTAIGNVAKTIGMTKIAQKSGLSRPSLYKALSEGAKPQFSTIIKVL
KAIGGQVNINPI

Nucleotide

• Length: 279 bp

>AT75689 NZ_CP020918:1874951-1875229 [Flavobacterium faecale]
ATGGAAACATCAAAATTTGACATATCGGATTATTTAGACAGTAATGAAATGATTGCTGCATATTTAAATGAAGTTTTAGA
AAATGGAGACGAAACTGACCTAGTTACAGCAATAGGAAATGTAGCTAAAACAATTGGAATGACTAAAATTGCTCAAAAAT
CTGGATTGAGTAGACCAAGTTTGTACAAAGCGCTATCTGAAGGAGCTAAACCGCAATTCTCAACAATTATAAAAGTTCTA
AAAGCAATAGGTGGTCAAGTAAATATTAATCCCATCTAA