Detailed information of TA system TA075552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 41975..42711
• Replicon: plasmid pKPN1481-2
• Accession: NZ_CP020851
• Organism: Klebsiella variicola strain KPN1481

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: B8O08_RS29880
• Protein ID: WP_086893249.1
• Coordinates: 41975..42457 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: B8O08_RS29885
• Protein ID: WP_003026799.1
• Coordinates: 42445..42711 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8O08_RS29855	37144..37292	-	149	Protein_32	aldo/keto reductase	-
B8O08_RS31245	37299..37457	-	159	Protein_33	Bcr/CflA family drug resistance efflux transporter	-
B8O08_RS29860	37695..38090	-	396	WP_048268429.1	helix-turn-helix domain-containing protein	-
B8O08_RS29865	38139..39485	-	1347	WP_077264124.1	ISNCY family transposase	-
B8O08_RS29870	39711..40343	+	633	WP_001567369.1	hypothetical protein	-
B8O08_RS29875	40372..41775	-	1404	WP_086893248.1	ISNCY-like element ISKpn21 family transposase	-
B8O08_RS29880	41975..42457	-	483	WP_086893249.1	GNAT family N-acetyltransferase	Toxin
B8O08_RS29885	42445..42711	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
B8O08_RS29890	42956..43390	-	435	WP_001567367.1	cell envelope integrity protein TolA	-
B8O08_RS29895	43437..43985	-	549	WP_001567366.1	thioredoxin fold domain-containing protein	-
B8O08_RS29900	44200..47085	-	2886	WP_086893250.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id	-	1..161100	161100
	-	inside	IScluster/Tn	aph(3'')-Ib / aph(6)-Id	-	38139..50765	12626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17296.98 Da
• Isoelectric Point: 8.7400

>T75552 WP_086893249.1 NZ_CP020851:c42457-41975 [Klebsiella variicola]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKVSQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T75552 NZ_CP020851:c42457-41975 [Klebsiella variicola]
GTGGGATGTATAACAGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGAGAAACGGTACTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAGGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATATCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGCTTCTATGCTCACCATGGATTTAAGGTATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT75552 WP_003026799.1 NZ_CP020851:c42711-42445 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT75552 NZ_CP020851:c42711-42445 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66