Detailed information of TA system TA075465

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 47199..47920
• Replicon: chromosome
• Accession: NZ_CP020835
• Organism: Escherichia coli strain CFSAN051542

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: BCD20_RS00270
• Protein ID: WP_047399350.1
• Coordinates: 47199..47540 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0A0FFR4
• Locus tag: BCD20_RS00275
• Protein ID: WP_045265421.1
• Coordinates: 47576..47920 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCD20_RS00250	43170..44354	-	1185	WP_044864163.1	sugar efflux transporter	-
BCD20_RS00260	45126..46163	-	1038	WP_045265419.1	virulence RhuM family protein	-
BCD20_RS00265	46251..47084	-	834	WP_047399701.1	DUF4942 domain-containing protein	-
BCD20_RS00270	47199..47540	-	342	WP_047399350.1	TA system toxin CbtA family protein	Toxin
BCD20_RS00275	47576..47920	-	345	WP_045265421.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BCD20_RS00280	47944..48039	-	96	WP_085381268.1	DUF987 family protein	-
BCD20_RS00285	48102..49315	+	1214	WP_139371347.1	IS3 family transposase	-
BCD20_RS25770	49197..49478	-	282	WP_157108322.1	DUF987 family protein	-
BCD20_RS00295	49489..49962	-	474	WP_025376489.1	DNA repair protein RadC	-
BCD20_RS00300	50033..50854	-	822	WP_047399357.1	DUF945 domain-containing protein	-
BCD20_RS00305	50923..51378	-	456	WP_047399359.1	hypothetical protein	-
BCD20_RS00315	51610..52413	-	804	WP_085381269.1	hypothetical protein	-
BCD20_RS00320	52433..52867	-	435	WP_085381270.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (14-113)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12748.67 Da
• Isoelectric Point: 8.5196

>T75465 WP_047399350.1 NZ_CP020835:c47540-47199 [Escherichia coli]
MNTSPATNQRVAKPCPSPVAVWQMLLTRLLAQHYGLALSDTPFCDETVIQEHINAGVTLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQAMGLLRQGHNLSTR

Nucleotide

• Length: 342 bp

>T75465 NZ_CP020835:c47540-47199 [Escherichia coli]
ATGAACACCTCTCCTGCAACAAATCAGCGGGTAGCGAAGCCATGCCCGTCGCCTGTGGCCGTCTGGCAGATGTTACTGAC
CCGTCTACTTGCACAACACTATGGCCTGGCCCTGAGTGACACGCCATTCTGCGATGAAACAGTCATTCAGGAACATATCA
ACGCAGGCGTTACTCTTGCCGATGCCGTGAACTTTCTGGTGGAAAAATACGAACTCGTTCGCATCGATCGCAAAGGATTT
AGCTGGCAGGAACAATCGCCTTATCTGCGGGCTGTAGACATTTTGCGGGCGCGACAGGCAATGGGGTTGTTACGACAAGG
CCATAATCTTTCGACACGCTGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12867.88 Da
• Isoelectric Point: 8.1459

>AT75465 WP_045265421.1 NZ_CP020835:c47920-47576 [Escherichia coli]
MKKDTANEVVDTLRWGLKCDITPHFGARLVQEGNRLHYLADRASIIGPFSKADLRHLNQAFPLLMKQMELMLTSGELNSR
HQHCVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 345 bp

>AT75465 NZ_CP020835:c47920-47576 [Escherichia coli]
ATGAAAAAAGACACAGCCAATGAAGTAGTGGATACGCTGCGATGGGGACTGAAATGCGACATCACACCACATTTCGGGGC
CCGTCTGGTGCAGGAAGGCAACCGCCTGCATTACCTCGCCGATCGGGCCAGCATAATCGGTCCGTTCAGCAAAGCTGATT
TACGCCATCTCAACCAGGCTTTCCCCTTGTTGATGAAGCAAATGGAGCTCATGCTCACCAGCGGAGAACTTAATTCCCGG
CACCAGCACTGCGTCACGCTGTATGCGAAAGGATTAACCTGTGAAGCCGATACGCTCGGCTCTTGTGGCTACGTCTATAT
CGCCATTTACCCCACTCAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0A0FFR4