Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 376410..376863 Replicon chromosome
Accession NZ_CP020753
Organism Shigella flexneri 1c strain Y394

Toxin (Protein)


Gene name itaT Uniprot ID A0A822PL35
Locus tag B7485_RS01825 Protein ID WP_005053055.1
Coordinates 376410..376568 (-) Length 53 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag B7485_RS01830 Protein ID WP_005053053.1
Coordinates 376552..376863 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7485_RS01810 (372969) 372969..375122 - 2154 WP_128882010.1 SEL1-like repeat protein -
B7485_RS01815 (375273) 375273..375970 + 698 WP_223368647.1 IS1 family transposase -
B7485_RS01820 (376018) 376018..376359 - 342 WP_005125115.1 GNAT family N-acetyltransferase -
B7485_RS01825 (376410) 376410..376568 - 159 WP_005053055.1 hypothetical protein Toxin
B7485_RS01830 (376552) 376552..376863 - 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
B7485_RS01835 (377048) 377048..377938 - 891 WP_000971346.1 heme o synthase -
B7485_RS01840 (377950) 377950..378279 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
B7485_RS01845 (378279) 378279..378893 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
B7485_RS01850 (378883) 378883..380874 - 1992 Protein_367 cytochrome o ubiquinol oxidase subunit I -
B7485_RS01855 (380896) 380896..381393 - 498 Protein_368 COX aromatic rich motif-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 375467..375970 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 53 a.a.        Molecular weight: 5914.82 Da        Isoelectric Point: 6.9172

>T75381 WP_005053055.1 NZ_CP020753:c376568-376410 [Shigella flexneri 1c]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRATRIAKEKCA

Download         Length: 159 bp

>T75381 NZ_CP020753:c376568-376410 [Shigella flexneri 1c]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT75381 WP_005053053.1 NZ_CP020753:c376863-376552 [Shigella flexneri 1c]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT75381 NZ_CP020753:c376863-376552 [Shigella flexneri 1c]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PL35

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References