Detailed information of TA system TA075376

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 82264..82993
• Replicon: plasmid unnamed3
• Accession: NZ_CP020746
• Organism: Bacillus mycoides strain Gnyt1

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: B7492_RS32705
• Protein ID: WP_085313506.1
• Coordinates: 82264..82836 (-)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	82898..82993 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7492_RS32680	77741..78193	-	453	WP_085313501.1	CPBP family intramembrane metalloprotease	-
B7492_RS32685	78177..80318	-	2142	WP_085313502.1	type IA DNA topoisomerase	-
B7492_RS32690	80418..80786	-	369	WP_085313503.1	hypothetical protein	-
B7492_RS32695	80957..81388	-	432	WP_085313504.1	hypothetical protein	-
B7492_RS32700	81546..82190	-	645	WP_085313505.1	hypothetical protein	-
B7492_RS32705	82264..82836	-	573	WP_085313506.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
-	82898..82993	-	96	NuclAT_0	-	Antitoxin
-	82898..82993	-	96	NuclAT_0	-	Antitoxin
-	82898..82993	-	96	NuclAT_0	-	Antitoxin
-	82898..82993	-	96	NuclAT_0	-	Antitoxin
B7492_RS32710	83071..84093	-	1023	WP_085313507.1	DUF3991 and TOPRIM domain-containing protein	-
B7492_RS32715	84165..84716	-	552	WP_085313508.1	metal-dependent hydrolase	-
B7492_RS32720	84738..85724	-	987	WP_085313509.1	hypothetical protein	-
B7492_RS32725	85788..86960	-	1173	WP_085313510.1	hypothetical protein	-
B7492_RS32730	86957..87166	-	210	WP_085313511.1	DUF5511 family protein	-
B7492_RS32735	87203..87859	-	657	WP_085313512.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..102550	102550

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22378.66 Da
• Isoelectric Point: 9.1862

>T75376 WP_085313506.1 NZ_CP020746:c82836-82264 [Bacillus mycoides]
MANKDNPKFYTISTKYIDYLRETDSKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNLNSQVSVKLFDIDNPEKR
IGVLLVNNMIPVPEKECKEIDIAEKTETDPKYGNLMLKQYLFLKENMDRVSNKVEKVYKDVTVQGKPSHKQKFLKGVCCD
FSKLEEKCQEYKEKDQARRIAYMRQLGRER

Nucleotide

• Length: 573 bp

>T75376 NZ_CP020746:c82836-82264 [Bacillus mycoides]
TTGGCTAATAAAGATAATCCTAAATTTTACACTATTTCAACTAAATATATAGACTATCTAAGAGAAACAGATAGTAAAGT
ACCATTTAATAAAGATGAACAGCATAGTAGACCGTATGTAGGTGTATTGGAAAAAATAAATGGTCATGATTATTTTGTAC
CTTTAACATCACGTAATGATAAAAATCTTAATAGCCAGGTCAGTGTAAAGTTATTTGATATTGATAACCCTGAGAAGCGA
ATTGGAGTGTTATTGGTCAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAACGGA
AACAGATCCTAAGTATGGTAATTTGATGCTAAAACAGTATTTGTTTTTAAAAGAGAATATGGATCGAGTTTCTAATAAAG
TGGAAAAAGTATATAAAGATGTAACTGTACAAGGAAAGCCTTCACATAAACAGAAATTCTTAAAAGGAGTTTGCTGTGAT
TTCTCTAAATTAGAGGAAAAGTGCCAGGAATATAAAGAAAAGGATCAAGCTAGAAGAATAGCATATATGCGCCAATTGGG
GCGTGAGAGATAA

Antitoxin

Nucleotide

• Length: 96 bp

>AT75376 NZ_CP020746:c82993-82898 [Bacillus mycoides]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCACAAAAAA

Structures

Antitoxin