Detailed information of TA system TA075154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 31083..31741
• Replicon: plasmid pSSMA17_1
• Accession: NZ_CP020580
• Organism: Acinetobacter baumannii strain SSMA17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B7L43_RS00220
• Protein ID: WP_000312250.1
• Coordinates: 31083..31442 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B7L43_RS00225
• Protein ID: WP_001096429.1
• Coordinates: 31442..31741 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7L43_RS00180	26406..26720	-	315	WP_000708715.1	hypothetical protein	-
B7L43_RS00190	27586..27921	-	336	WP_000653925.1	hypothetical protein	-
B7L43_RS00195	27973..28731	-	759	WP_001053128.1	hypothetical protein	-
B7L43_RS00200	28798..29181	-	384	WP_000654348.1	hypothetical protein	-
B7L43_RS00205	29320..30018	-	699	WP_000873188.1	hypothetical protein	-
B7L43_RS00210	30085..30267	-	183	WP_000373385.1	hypothetical protein	-
B7L43_RS00215	30316..30882	-	567	WP_000710385.1	hypothetical protein	-
B7L43_RS00220	31083..31442	+	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7L43_RS00225	31442..31741	+	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
B7L43_RS00235	32277..32813	-	537	WP_000732006.1	hypothetical protein	-
B7L43_RS00240	32863..33417	-	555	WP_000790082.1	hypothetical protein	-
B7L43_RS00250	33653..34381	-	729	WP_001022819.1	hypothetical protein	-
B7L43_RS00255	34418..34759	-	342	WP_002001471.1	hypothetical protein	-
B7L43_RS00260	34803..35315	-	513	WP_000840065.1	hypothetical protein	-
B7L43_RS00265	35397..35906	-	510	WP_001043200.1	hypothetical protein	-
B7L43_RS00270	35946..36575	-	630	WP_000701002.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90973	90973

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T75154 WP_000312250.1 NZ_CP020580:31083-31442 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T75154 NZ_CP020580:31083-31442 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGTCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTATGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT75154 WP_001096429.1 NZ_CP020580:31442-31741 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT75154 NZ_CP020580:31442-31741 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA