Detailed information of TA system TA075146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 31133..31791
• Replicon: plasmid p15A5_1
• Accession: NZ_CP020573
• Organism: Acinetobacter baumannii strain 15A5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B6S64_RS00215
• Protein ID: WP_000312250.1
• Coordinates: 31133..31492 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B6S64_RS00220
• Protein ID: WP_001096429.1
• Coordinates: 31492..31791 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6S64_RS00175	26457..26771	-	315	WP_000708713.1	hypothetical protein	-
B6S64_RS00185	27636..27971	-	336	WP_000654055.1	hypothetical protein	-
B6S64_RS00190	28023..28781	-	759	WP_060853650.1	hypothetical protein	-
B6S64_RS00195	28848..29231	-	384	WP_000654347.1	hypothetical protein	-
B6S64_RS00200	29370..30068	-	699	WP_000873188.1	hypothetical protein	-
B6S64_RS00205	30135..30317	-	183	WP_000373384.1	hypothetical protein	-
B6S64_RS00210	30366..30932	-	567	WP_031948745.1	hypothetical protein	-
B6S64_RS00215	31133..31492	+	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B6S64_RS00220	31492..31791	+	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
B6S64_RS00230	32328..32864	-	537	WP_060853649.1	hypothetical protein	-
B6S64_RS19590	32914..33467	-	554	Protein_46	hypothetical protein	-
B6S64_RS00245	33702..34430	-	729	WP_001022819.1	hypothetical protein	-
B6S64_RS00250	34467..34808	-	342	WP_002001471.1	hypothetical protein	-
B6S64_RS00255	34852..35364	-	513	WP_000840065.1	hypothetical protein	-
B6S64_RS00260	35446..35955	-	510	WP_001043199.1	hypothetical protein	-
B6S64_RS00265	35995..36624	-	630	WP_002081923.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..74241	74241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T75146 WP_000312250.1 NZ_CP020573:31133-31492 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T75146 NZ_CP020573:31133-31492 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT75146 WP_001096429.1 NZ_CP020573:31492-31791 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT75146 NZ_CP020573:31492-31791 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA