Detailed information of TA system TA075101

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3340313..3341148
• Replicon: chromosome
• Accession: NZ_CP020545
• Organism: Escherichia coli strain ZJ3920

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: B6V57_RS17385
• Protein ID: WP_148123943.1
• Coordinates: 3340313..3340690 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0K4A940
• Locus tag: B6V57_RS17390
• Protein ID: WP_001285576.1
• Coordinates: 3340780..3341148 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6V57_RS17355	3335955..3337796	+	1842	WP_000437380.1	RNA polymerase sigma factor RpoD	-
B6V57_RS17360	3337875..3338381	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
B6V57_RS17370	3338681..3339526	-	846	WP_001280427.1	DUF4942 domain-containing protein	-
B6V57_RS17375	3339611..3339808	-	198	WP_000445281.1	DUF957 domain-containing protein	-
B6V57_RS17380	3339828..3340316	-	489	WP_000761669.1	hypothetical protein	-
B6V57_RS17385	3340313..3340690	-	378	WP_148123943.1	TA system toxin CbtA family protein	Toxin
B6V57_RS17390	3340780..3341148	-	369	WP_001285576.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B6V57_RS17395	3341228..3341449	-	222	WP_000692176.1	DUF987 domain-containing protein	-
B6V57_RS17400	3341536..3342012	-	477	WP_001360063.1	RadC family protein	-
B6V57_RS17410	3342028..3342513	-	486	WP_000206656.1	antirestriction protein	-
B6V57_RS17415	3342605..3343423	-	819	WP_021520251.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14131.20 Da
• Isoelectric Point: 8.2904

>T75101 WP_148123943.1 NZ_CP020545:c3340690-3340313 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQILLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTRSQLINSIDILRARRATGLMVRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T75101 NZ_CP020545:c3340690-3340313 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGTCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13599.36 Da
• Isoelectric Point: 6.3159

>AT75101 WP_001285576.1 NZ_CP020545:c3341148-3340780 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT75101 NZ_CP020545:c3341148-3340780 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGTGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTCAGCCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0K4A940