Detailed information of TA system TA075026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5058022..5058624
• Replicon: chromosome
• Accession: NZ_CP020525
• Organism: Enterobacter cloacae strain 109

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: AO413_RS25045
• Protein ID: WP_022649837.1
• Coordinates: 5058313..5058624 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AO413_RS25040
• Protein ID: WP_022649836.1
• Coordinates: 5058022..5058312 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO413_RS25025	5055520..5056422	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
AO413_RS25030	5056419..5057054	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
AO413_RS25035	5057051..5057980	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
AO413_RS25040	5058022..5058312	-	291	WP_022649836.1	helix-turn-helix domain-containing protein	Antitoxin
AO413_RS25045	5058313..5058624	-	312	WP_022649837.1	hypothetical protein	Toxin
AO413_RS25050	5058773..5059714	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
AO413_RS25055	5059759..5060196	-	438	WP_022649839.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AO413_RS25060	5060193..5061074	-	882	WP_022649840.1	virulence factor BrkB family protein	-
AO413_RS25065	5061068..5061667	-	600	WP_022649841.1	glucose-1-phosphatase	-
AO413_RS25070	5061786..5062586	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
AO413_RS25075	5062621..5063517	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5048242..5058624	10382

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T75026 WP_022649837.1 NZ_CP020525:c5058624-5058313 [Enterobacter cloacae]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T75026 NZ_CP020525:c5058624-5058313 [Enterobacter cloacae]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT75026 WP_022649836.1 NZ_CP020525:c5058312-5058022 [Enterobacter cloacae]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT75026 NZ_CP020525:c5058312-5058022 [Enterobacter cloacae]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6