Detailed information of TA system TA075024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 4714002..4714903
• Replicon: chromosome
• Accession: NZ_CP020525
• Organism: Enterobacter cloacae strain 109

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A330G4J7
• Locus tag: AO413_RS23365
• Protein ID: WP_022652120.1
• Coordinates: 4714445..4714903 (+)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: AO413_RS23360
• Protein ID: WP_022652121.1
• Coordinates: 4714002..4714448 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO413_RS23325	4709494..4709871	+	378	WP_015063074.1	hypothetical protein	-
AO413_RS23330	4710007..4710441	+	435	WP_015063073.1	hypothetical protein	-
AO413_RS23335	4710442..4710762	+	321	WP_077258061.1	hypothetical protein	-
AO413_RS23340	4710878..4711513	+	636	WP_022652125.1	hypothetical protein	-
AO413_RS23345	4711575..4712186	+	612	WP_022652124.1	hypothetical protein	-
AO413_RS23350	4712173..4713441	+	1269	WP_022652123.1	site-specific DNA-methyltransferase	-
AO413_RS23355	4713617..4713925	+	309	WP_022652122.1	hypothetical protein	-
AO413_RS23360	4714002..4714448	+	447	WP_022652121.1	DUF2384 domain-containing protein	Antitoxin
AO413_RS23365	4714445..4714903	+	459	WP_022652120.1	RES family NAD+ phosphorylase	Toxin
AO413_RS23370	4714941..4715171	+	231	WP_022652119.1	hypothetical protein	-
AO413_RS23375	4715506..4715838	+	333	WP_022652118.1	hypothetical protein	-
AO413_RS23380	4715895..4716158	+	264	WP_032610382.1	hypothetical protein	-
AO413_RS23385	4716388..4717194	+	807	WP_015063028.1	hypothetical protein	-
AO413_RS23390	4717241..4717687	+	447	WP_015063027.1	hypothetical protein	-
AO413_RS23395	4717865..4718812	+	948	Protein_4456	phosphoadenosine phosphosulfate reductase family protein	-
AO413_RS23400	4718948..4719583	-	636	WP_012477419.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16758.14 Da
• Isoelectric Point: 4.4843

>T75024 WP_022652120.1 NZ_CP020525:4714445-4714903 [Enterobacter cloacae]
VILYRMTKTRYITTAWSGLGAKEAGGRWNSVGTALVYLSETASLTMLETLVHINAPQLLDAYTLLSIDVPDDQIQAFDLN
LLPPGWASEDAPAELALYGDNWAESGSSVALRIPSALSPVEFNYLLNPAHPAIFDLMKTVQKIPYQYDTRLK

Nucleotide

• Length: 459 bp

>T75024 NZ_CP020525:4714445-4714903 [Enterobacter cloacae]
GTGATCCTGTATCGGATGACCAAGACCCGCTATATTACGACTGCATGGTCGGGATTAGGTGCGAAAGAAGCGGGAGGCCG
CTGGAACAGTGTCGGCACAGCACTTGTCTATCTGTCCGAGACGGCCTCTCTGACCATGCTGGAGACGCTGGTGCATATCA
ATGCCCCGCAGCTTCTGGACGCTTACACGCTGCTGAGCATTGATGTCCCGGATGACCAGATCCAGGCCTTTGACTTGAAC
CTGCTGCCGCCGGGCTGGGCAAGTGAAGATGCGCCCGCAGAACTTGCCCTGTATGGCGATAACTGGGCTGAAAGTGGCTC
TTCTGTTGCCCTGCGTATTCCCAGTGCATTATCTCCGGTGGAGTTTAACTACCTTTTAAATCCGGCTCATCCGGCGATTT
TTGATCTGATGAAAACCGTCCAGAAAATTCCTTACCAGTACGACACACGGCTGAAATAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16606.03 Da
• Isoelectric Point: 7.4048

>AT75024 WP_022652121.1 NZ_CP020525:4714002-4714448 [Enterobacter cloacae]
MTMKVFSPDLSKMNPHGLWQVVGLDNDGIALMDKISHGLEGKVAHSISEWANITPSELRKMSGIPNTTFNRSVKARFTAD
QSERLVRIIRVIERAVELFEGDKEAAQKWMNEPNRGLSWKTPADVVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT75024 NZ_CP020525:4714002-4714448 [Enterobacter cloacae]
ATGACTATGAAAGTTTTTTCTCCAGATTTATCCAAAATGAACCCGCACGGCTTATGGCAGGTGGTGGGTCTGGATAATGA
CGGCATTGCGCTGATGGACAAAATTAGCCACGGACTGGAAGGTAAAGTCGCTCATTCCATTTCTGAATGGGCGAACATCA
CGCCGTCGGAACTGCGCAAAATGTCCGGCATTCCGAACACCACCTTCAATCGCAGCGTAAAAGCCCGTTTCACTGCGGAT
CAGAGCGAACGTCTGGTGCGTATTATTCGCGTCATTGAACGGGCTGTAGAGCTGTTCGAAGGCGACAAAGAGGCAGCACA
GAAATGGATGAACGAGCCAAACCGTGGCTTGAGCTGGAAGACTCCGGCAGATGTTGTCTCTTCGGAAACCGGCGCACTCG
AAGTGATGCGCCTGATCACCCGTATTGAACACGGAGTCTATTCGTGA

Structures

Toxin

• AlphaFold DB: A0A330G4J7