Detailed information of TA system TA074967

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4664142..4664977
• Replicon: chromosome
• Accession: NZ_CP020516
• Organism: Escherichia coli strain 222

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J1YPH7
• Locus tag: A9P13_RS24295
• Protein ID: WP_029701676.1
• Coordinates: 4664600..4664977 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0K5N986
• Locus tag: A9P13_RS24290
• Protein ID: WP_021553056.1
• Coordinates: 4664142..4664510 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P13_RS24255	4659804..4660484	+	681	WP_029702103.1	WYL domain-containing protein	-
A9P13_RS24260	4660632..4661309	+	678	WP_001097302.1	hypothetical protein	-
A9P13_RS24265	4661315..4661548	+	234	WP_001278293.1	DUF905 family protein	-
A9P13_RS24270	4661638..4662456	+	819	WP_001175165.1	DUF945 domain-containing protein	-
A9P13_RS24275	4662722..4663201	+	480	WP_001564060.1	antirestriction protein	-
A9P13_RS24280	4663217..4663693	+	477	WP_021553055.1	RadC family protein	-
A9P13_RS24285	4663758..4663979	+	222	WP_000692345.1	DUF987 domain-containing protein	-
A9P13_RS24290	4664142..4664510	+	369	WP_021553056.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A9P13_RS24295	4664600..4664977	+	378	WP_029701676.1	TA system toxin CbtA family protein	Toxin
A9P13_RS28800	4664974..4665123	+	150	Protein_4436	hypothetical protein	-
A9P13_RS24305	4665202..4665396	+	195	WP_024181941.1	DUF957 domain-containing protein	-
A9P13_RS24310	4665481..4666323	+	843	WP_022645115.1	DUF4942 domain-containing protein	-
A9P13_RS24320	4667072..4668610	+	1539	WP_001187196.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4630604..4676422	45818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14066.04 Da
• Isoelectric Point: 7.8045

>T74967 WP_029701676.1 NZ_CP020516:4664600-4664977 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T74967 NZ_CP020516:4664600-4664977 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13615.39 Da
• Isoelectric Point: 7.0264

>AT74967 WP_021553056.1 NZ_CP020516:4664142-4664510 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT74967 NZ_CP020516:4664142-4664510 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J1YPH7

Antitoxin

• AlphaFold DB: A0A0K5N986