Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 586392..587028 Replicon chromosome
Accession NZ_CP020352
Organism Bacillus paralicheniformis strain MDJK30

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag BLMD_RS02980 Protein ID WP_003179128.1
Coordinates 586678..587028 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag BLMD_RS02975 Protein ID WP_006638778.1
Coordinates 586392..586673 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BLMD_RS02955 581502..582986 + 1485 WP_039073238.1 PH domain-containing protein -
BLMD_RS02960 582983..583582 - 600 WP_048349919.1 rhomboid family intramembrane serine protease -
BLMD_RS02965 583926..584885 + 960 WP_164468292.1 outer membrane lipoprotein carrier protein LolA -
BLMD_RS02970 585111..586280 + 1170 WP_196220021.1 alanine racemase -
BLMD_RS02975 586392..586673 + 282 WP_006638778.1 hypothetical protein Antitoxin
BLMD_RS02980 586678..587028 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BLMD_RS02985 587146..587973 + 828 WP_023857863.1 RsbT co-antagonist protein RsbRA -
BLMD_RS02990 587977..588342 + 366 WP_031305225.1 STAS domain-containing protein -
BLMD_RS02995 588345..588746 + 402 WP_023857861.1 anti-sigma regulatory factor -
BLMD_RS03000 588757..589764 + 1008 WP_020450261.1 PP2C family protein-serine/threonine phosphatase -
BLMD_RS03005 589823..590149 + 327 WP_020450262.1 anti-sigma factor antagonist -
BLMD_RS03010 590149..590631 + 483 WP_020450263.1 anti-sigma B factor RsbW -
BLMD_RS03015 590597..591388 + 792 WP_023857858.1 RNA polymerase sigma factor SigB -
BLMD_RS03020 591385..591984 + 600 WP_023857857.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T74354 WP_003179128.1 NZ_CP020352:586678-587028 [Bacillus paralicheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T74354 NZ_CP020352:586678-587028 [Bacillus paralicheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT74354 WP_006638778.1 NZ_CP020352:586392-586673 [Bacillus paralicheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT74354 NZ_CP020352:586392-586673 [Bacillus paralicheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATTAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References