Detailed information of TA system TA074353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5772573..5773182
• Replicon: chromosome
• Accession: NZ_CP020351
• Organism: Pseudomonas amygdali pv. lachrymans strain NM002

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: B5U27_RS27470
• Protein ID: WP_080164823.1
• Coordinates: 5772573..5772953 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A3M6GEZ6
• Locus tag: B5U27_RS27475
• Protein ID: WP_005746660.1
• Coordinates: 5772943..5773182 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5U27_RS27440	5767717..5768247	+	531	WP_044299855.1	nucleotidyltransferase family protein	-
B5U27_RS27445	5768477..5769091	-	615	WP_005746667.1	histidine phosphatase family protein	-
B5U27_RS27450	5769195..5769536	-	342	WP_005746665.1	hypothetical protein	-
B5U27_RS27455	5769726..5770148	+	423	WP_005746664.1	DUF1493 family protein	-
B5U27_RS27460	5770700..5771785	+	1086	WP_005746663.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
B5U27_RS27465	5771929..5772666	+	738	WP_005746662.1	hypothetical protein	-
B5U27_RS27470	5772573..5772953	-	381	WP_080164823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B5U27_RS27475	5772943..5773182	-	240	WP_005746660.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
B5U27_RS27480	5773346..5773819	-	474	WP_005746659.1	GNAT family N-acetyltransferase	-
B5U27_RS27485	5773920..5774468	-	549	WP_005746658.1	GNAT family N-acetyltransferase	-
B5U27_RS27490	5774628..5775122	+	495	WP_164513123.1	hypothetical protein	-
B5U27_RS27495	5775156..5776430	-	1275	WP_004667253.1	OprD family porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-70)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13873.07 Da
• Isoelectric Point: 8.6903

>T74353 WP_080164823.1 NZ_CP020351:c5772953-5772573 [Pseudomonas amygdali pv. lachrymans]
MTYNLELDARALKEWHKLGDTVRQQLKKKLATILVAPRVEANRLHALPDCYKIKLRSSGYRLVYQVIDQEVVVFVVAVDK
RECPPATADESLTGVDQPSVAAFLAARAASIAASVSWVCSSPLYAS

Nucleotide

• Length: 381 bp

>T74353 NZ_CP020351:c5772953-5772573 [Pseudomonas amygdali pv. lachrymans]
ATGACCTATAACCTCGAACTCGATGCGCGGGCTCTAAAGGAATGGCACAAATTAGGAGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCTCCGCGTGTTGAAGCCAATCGTCTGCATGCATTGCCTGACTGCTACAAAATCA
AACTGCGCAGTAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAAGAGGTTGTGGTCTTCGTGGTTGCGGTAGACAAG
CGTGAATGTCCGCCCGCAACGGCTGACGAGTCGCTGACTGGTGTTGATCAACCCTCAGTGGCGGCCTTTTTGGCCGCACG
TGCAGCGAGCATCGCCGCTTCCGTTTCCTGGGTGTGCAGCTCACCTTTGTACGCCAGTTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8999.40 Da
• Isoelectric Point: 4.6017

>AT74353 WP_005746660.1 NZ_CP020351:c5773182-5772943 [Pseudomonas amygdali pv. lachrymans]
MASINVRIDDDLKARAYLELEKLGVTPSELLRQTLQYVAERGQLPFKTVLMTEEDEALLATVRERLATPQRVKISLDDL

Nucleotide

• Length: 240 bp

>AT74353 NZ_CP020351:c5773182-5772943 [Pseudomonas amygdali pv. lachrymans]
ATGGCGTCTATCAACGTTCGTATCGACGACGATCTCAAAGCGCGTGCTTACCTCGAGCTGGAAAAGCTTGGCGTCACCCC
CTCGGAGTTGCTGCGCCAAACCCTGCAGTACGTAGCTGAGCGTGGTCAGCTACCCTTCAAGACGGTTCTGATGACAGAGG
AAGACGAAGCGCTGTTGGCAACTGTGCGTGAGCGCCTTGCAACTCCGCAGCGGGTCAAGATTTCACTGGATGACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A3M6GEZ6