Detailed information of TA system TA074245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2573901..2574442
• Replicon: chromosome
• Accession: NZ_CP020335
• Organism: Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B0X70_RS11330
• Protein ID: WP_046394614.1
• Coordinates: 2573901..2574227 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: B0X70_RS11335
• Protein ID: WP_217471513.1
• Coordinates: 2574224..2574442 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0X70_RS11325 (B0X70_11440)	2568906..2573783	+	4878	WP_217471512.1	RHS repeat-associated core domain-containing protein	-
B0X70_RS11330 (B0X70_11445)	2573901..2574227	-	327	WP_046394614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B0X70_RS11335 (B0X70_11450)	2574224..2574442	-	219	WP_217471513.1	antitoxin MazE family protein	Antitoxin
B0X70_RS11340 (B0X70_11455)	2574905..2575441	+	537	WP_217471514.1	hypothetical protein	-
B0X70_RS11345 (B0X70_11460)	2575564..2576805	-	1242	WP_246593532.1	MFS transporter	-
B0X70_RS11350 (B0X70_11465)	2576950..2577864	+	915	WP_217471516.1	LysR family transcriptional regulator	-
B0X70_RS11355 (B0X70_11470)	2577995..2579206	+	1212	WP_217471517.1	ROK family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (6-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11836.93 Da
• Isoelectric Point: 6.9774

>T74245 WP_046394614.1 NZ_CP020335:c2574227-2573901 [Photorhabdus akhurstii]
VKRGDLITVALQGAYGKPRPALVIQSDNFDEHPSVTILPVTSTLIDAPLFRVMIEPLPETNLEKPSQVMIDKTMTLPKEK
AGAVFGKLDYATMQQVDRCLALFLGIAK

Nucleotide

• Length: 327 bp

>T74245 NZ_CP020335:c2574227-2573901 [Photorhabdus akhurstii]
GTGAAACGGGGAGACTTGATAACAGTTGCTCTACAAGGGGCTTATGGCAAGCCCCGCCCTGCGCTGGTTATCCAATCTGA
CAACTTTGATGAACATCCCAGTGTTACTATTTTGCCCGTGACCTCAACATTAATTGATGCACCACTTTTCCGGGTGATGA
TCGAGCCTCTTCCTGAAACAAATCTTGAAAAACCGTCACAGGTCATGATTGATAAAACCATGACTCTCCCTAAAGAAAAA
GCTGGCGCAGTATTTGGTAAACTCGATTACGCGACGATGCAACAAGTGGATCGATGTTTAGCTTTGTTTTTAGGTATCGC
AAAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8513.70 Da
• Isoelectric Point: 9.1421

>AT74245 WP_217471513.1 NZ_CP020335:c2574442-2574224 [Photorhabdus akhurstii]
MNKVADRVKKYRQQLRMSGLRPVQIWVPDTRLPHFKEECRRQSRLAMATQDKETNTLLENAINELADSGDWE

Nucleotide

• Length: 219 bp

>AT74245 NZ_CP020335:c2574442-2574224 [Photorhabdus akhurstii]
ATGAATAAGGTTGCAGATCGTGTCAAAAAATACAGACAACAGCTCAGGATGTCAGGATTAAGGCCCGTTCAAATATGGGT
GCCTGATACACGCCTTCCTCATTTTAAAGAGGAGTGTCGGCGTCAAAGCCGTCTGGCTATGGCAACACAGGACAAGGAAA
CGAATACACTATTAGAAAACGCGATAAATGAACTAGCTGATTCAGGAGACTGGGAGTGA