Detailed information of TA system TA074244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE(toxin)
• Location: 2470211..2470733
• Replicon: chromosome
• Accession: NZ_CP020335
• Organism: Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: B0X70_RS10825
• Protein ID: WP_217471491.1
• Coordinates: 2470452..2470733 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0A0CW45
• Locus tag: B0X70_RS10820
• Protein ID: WP_036806437.1
• Coordinates: 2470211..2470462 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0X70_RS10785 (B0X70_10890)	2465841..2466404	+	564	WP_217471489.1	VOC family protein	-
B0X70_RS10790 (B0X70_10895)	2466420..2466734	-	315	WP_105395393.1	type II toxin-antitoxin system MqsA family antitoxin	-
B0X70_RS10795 (B0X70_10900)	2466735..2467070	-	336	WP_105395394.1	toxin	-
B0X70_RS10800 (B0X70_10905)	2467141..2467251	+	111	Protein_2080	VOC family protein	-
B0X70_RS10805 (B0X70_10910)	2467419..2468165	+	747	WP_046395062.1	copper homeostasis protein CutC	-
B0X70_RS10810 (B0X70_10915)	2468206..2469177	-	972	WP_217471490.1	tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB	-
B0X70_RS10815 (B0X70_10920)	2469174..2469932	-	759	WP_046395065.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
B0X70_RS10820 (B0X70_10925)	2470211..2470462	+	252	WP_036806437.1	plasmid stabilization protein	Antitoxin
B0X70_RS10825 (B0X70_10930)	2470452..2470733	+	282	WP_217471491.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B0X70_RS10830 (B0X70_10935)	2470863..2471258	-	396	WP_036775538.1	MAPEG family protein	-
B0X70_RS10835 (B0X70_10940)	2471490..2473262	+	1773	WP_217471716.1	aspartate--tRNA ligase	-
B0X70_RS10840 (B0X70_10945)	2473262..2473699	+	438	WP_036806429.1	dihydroneopterin triphosphate diphosphatase	-
B0X70_RS10845 (B0X70_10950)	2473729..2474472	+	744	WP_036806426.1	YebC/PmpR family DNA-binding transcriptional regulator	-
B0X70_RS10850 (B0X70_10955)	2474582..2475103	+	522	WP_217471492.1	crossover junction endodeoxyribonuclease RuvC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10908.86 Da
• Isoelectric Point: 10.7600

>T74244 WP_217471491.1 NZ_CP020335:2470452-2470733 [Photorhabdus akhurstii]
MIYSLEFEKRALKEWKKSRHPIKEQLKKKLAERLENPHVPSARLRGRANRYKIKLRSSGYCLVYEVNDNKIILLVIAIGK
RAADAVYTLANER

Nucleotide

• Length: 282 bp

>T74244 NZ_CP020335:2470452-2470733 [Photorhabdus akhurstii]
ATGATCTATAGCCTGGAATTTGAAAAACGGGCCTTAAAAGAGTGGAAGAAGTCACGTCATCCAATTAAGGAGCAGCTAAA
GAAAAAATTGGCTGAACGCTTAGAAAACCCTCATGTACCGTCAGCAAGATTAAGAGGGCGAGCGAACCGATACAAAATTA
AATTGCGCTCTTCTGGCTACTGCTTAGTTTATGAAGTGAATGACAATAAGATTATCTTGCTGGTAATTGCTATTGGTAAA
CGGGCCGCAGATGCGGTTTATACCCTCGCCAATGAGAGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.41 Da
• Isoelectric Point: 3.7677

>AT74244 WP_036806437.1 NZ_CP020335:2470211-2470462 [Photorhabdus akhurstii]
MAYQILTNLAASITDLKRDPMGTYLQGEGEAVAILNRNEPAFYCVPPELFAYYQELAEDAALNKIADERLENAEFVSVNL
DDL

Nucleotide

• Length: 252 bp

>AT74244 NZ_CP020335:2470211-2470462 [Photorhabdus akhurstii]
ATGGCATATCAAATCTTAACAAACTTAGCGGCAAGTATTACCGATTTGAAACGTGACCCAATGGGGACATATTTGCAGGG
GGAAGGTGAAGCGGTCGCAATCCTGAATCGGAATGAACCTGCGTTTTATTGTGTGCCTCCCGAATTATTCGCCTACTATC
AGGAGCTAGCAGAGGATGCCGCACTGAACAAAATTGCAGATGAACGTTTAGAAAATGCTGAATTTGTTAGTGTGAACCTA
GATGATCTATAG

Structures

Antitoxin

• AlphaFold DB: A0A0A0CW45