Detailed information of TA system TA074103

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 872966..873803
• Replicon: chromosome
• Accession: NZ_CP020106
• Organism: Escherichia coli strain 13E0780

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: EC780_RS04670
• Protein ID: WP_000854687.1
• Coordinates: 873423..873803 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: EC780_RS04665
• Protein ID: WP_012816826.1
• Coordinates: 872966..873334 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC780_RS04625	869153..869830	+	678	WP_024226808.1	hypothetical protein	-
EC780_RS04630	869846..870256	+	411	WP_000848829.1	hypothetical protein	-
EC780_RS04640	870476..871297	+	822	WP_001234566.1	DUF945 domain-containing protein	-
EC780_RS04645	871297..871542	+	246	WP_000680588.1	hypothetical protein	-
EC780_RS04650	871636..872109	+	474	WP_001364892.1	antirestriction protein	-
EC780_RS04655	872125..872601	+	477	WP_001364966.1	RadC family protein	-
EC780_RS04660	872670..872891	+	222	WP_000691817.1	DUF987 domain-containing protein	-
EC780_RS04665	872966..873334	+	369	WP_012816826.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EC780_RS04670	873423..873803	+	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
EC780_RS04675	873800..874288	+	489	WP_032314654.1	hypothetical protein	-
EC780_RS04680	874308..874505	+	198	WP_000772026.1	DUF957 domain-containing protein	-
EC780_RS04685	874590..875435	+	846	WP_001274558.1	DUF4942 domain-containing protein	-
EC780_RS04695	875723..876229	+	507	WP_000228940.1	G/U mismatch-specific DNA glycosylase	-
EC780_RS04700	876307..878148	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T74103 WP_000854687.1 NZ_CP020106:873423-873803 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T74103 NZ_CP020106:873423-873803 [Escherichia coli]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13780.56 Da
• Isoelectric Point: 6.4763

>AT74103 WP_012816826.1 NZ_CP020106:872966-873334 [Escherichia coli]
VSDTLHETNYPNDNHNRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT74103 NZ_CP020106:872966-873334 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACAACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67