Detailed information of TA system TA073960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 198011..198747
• Replicon: plasmid unitig_2
• Accession: NZ_CP020069
• Organism: Klebsiella pneumoniae strain AR_0068

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: AM447_RS29210
• Protein ID: WP_003026803.1
• Coordinates: 198011..198493 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: AM447_RS29215
• Protein ID: WP_003026799.1
• Coordinates: 198481..198747 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM447_RS29190	193648..194610	+	963	WP_004152113.1	M48 family metalloprotease	-
AM447_RS30180	194698..195952	+	1255	Protein_192	IS3 family transposase	-
AM447_RS29205	196453..197799	-	1347	WP_020314316.1	ISNCY family transposase	-
AM447_RS29210	198011..198493	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
AM447_RS29215	198481..198747	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
AM447_RS29220	198923..199177	-	255	WP_004152108.1	hypothetical protein	-
AM447_RS29225	199253..199510	-	258	WP_004152107.1	hypothetical protein	-
AM447_RS29230	199559..199762	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
AM447_RS29235	199796..200164	-	369	WP_004152105.1	hypothetical protein	-
AM447_RS29240	200208..200702	-	495	WP_004152104.1	DNA-binding protein	-
AM447_RS29245	200733..201308	-	576	WP_004152103.1	hypothetical protein	-
AM447_RS29250	201296..201565	-	270	WP_004152102.1	hypothetical protein	-
AM447_RS29255	201923..202273	+	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
AM447_RS29260	202323..202685	+	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1	-	1..213013	213013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T73960 WP_003026803.1 NZ_CP020069:c198493-198011 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T73960 NZ_CP020069:c198493-198011 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT73960 WP_003026799.1 NZ_CP020069:c198747-198481 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT73960 NZ_CP020069:c198747-198481 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66