Detailed information of TA system TA073920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 319119..319753
• Replicon: chromosome
• Accession: NZ_CP020061
• Organism: Klebsiella pneumoniae strain AR_0117

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM364_RS01550
• Protein ID: WP_020801515.1
• Coordinates: 319349..319753 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AM364_RS01545
• Protein ID: WP_020801512.1
• Coordinates: 319119..319349 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM364_RS01535	315329..317191	+	1863	WP_020801509.1	hypothetical protein	-
AM364_RS01540	317268..318137	-	870	WP_020801506.1	integrase domain-containing protein	-
AM364_RS01545	319119..319349	+	231	WP_020801512.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AM364_RS01550	319349..319753	+	405	WP_020801515.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AM364_RS01555	319875..320750	-	876	WP_020801521.1	DNA adenine methylase	-
AM364_RS01560	320830..322563	-	1734	WP_020801533.1	ATP-binding protein	-
AM364_RS01565	323102..324115	-	1014	WP_020801500.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15004.34 Da
• Isoelectric Point: 7.8441

>T73920 WP_020801515.1 NZ_CP020061:319349-319753 [Klebsiella pneumoniae]
MLKYMLDTNICIYTIKNKPQAVREAFNQHYGRMCISSVTLMELIYGAEKSASPEKNLRVVEGFIARLEVLNYGIDAAVQT
GQIRAELARAGTPVGPYDSMIAAHARALGLILVTNNTREFERINGLRLEDWSIN

Nucleotide

• Length: 405 bp

>T73920 NZ_CP020061:319349-319753 [Klebsiella pneumoniae]
ATGCTCAAATACATGCTGGATACCAATATCTGTATCTATACGATCAAAAATAAACCTCAGGCAGTCCGGGAGGCGTTTAA
CCAGCATTACGGGCGGATGTGTATCAGTTCAGTCACGCTGATGGAGCTGATTTATGGCGCGGAAAAGTCCGCCAGCCCGG
AGAAAAACCTGCGCGTGGTCGAAGGATTTATCGCCCGGCTGGAGGTGCTGAATTATGGTATCGACGCGGCGGTACAGACG
GGGCAAATCCGGGCTGAACTGGCAAGAGCAGGTACGCCCGTTGGCCCGTATGACAGCATGATTGCGGCCCACGCCCGTGC
ATTGGGTTTAATTCTGGTGACGAACAACACCCGTGAGTTCGAACGTATTAATGGCTTACGCCTGGAAGACTGGAGTATCA
ACTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8495.46 Da
• Isoelectric Point: 4.7527

>AT73920 WP_020801512.1 NZ_CP020061:319119-319349 [Klebsiella pneumoniae]
MEKTTVFKSNRSQAVRLPKAVALPEDVKHVDIVAVGRTRIITPAGESWDSWFDGESVTSDFMATREQPGDQDREGF

Nucleotide

• Length: 231 bp

>AT73920 NZ_CP020061:319119-319349 [Klebsiella pneumoniae]
ATGGAAAAGACAACCGTATTCAAAAGCAACCGTAGCCAGGCGGTGCGGCTGCCAAAAGCGGTGGCTTTACCGGAGGATGT
CAAACATGTGGATATCGTTGCGGTAGGGCGAACGCGCATTATTACTCCCGCAGGCGAAAGCTGGGACAGCTGGTTTGATG
GTGAGAGCGTGACGTCTGACTTTATGGCTACCCGTGAGCAGCCCGGTGACCAGGACAGGGAAGGGTTCTGA