Detailed information of TA system TA073747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1124682..1125313
• Replicon: chromosome
• Accession: NZ_CP020013
• Organism: Haemophilus influenzae strain 6P32H1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q4QLW0
• Locus tag: BV140_RS05715
• Protein ID: WP_005651560.1
• Coordinates: 1124682..1125080 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BV140_RS05720
• Protein ID: WP_005686209.1
• Coordinates: 1125080..1125313 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BV140_RS05695	1119875..1120993	+	1119	WP_005686201.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-
BV140_RS05700	1120994..1122016	+	1023	WP_005655937.1	outer membrane-stress sensor serine endopeptidase DegS	-
BV140_RS05705	1122090..1122905	-	816	WP_005686204.1	DNA-formamidopyrimidine glycosylase	-
BV140_RS05710	1123127..1124662	-	1536	WP_005686206.1	L-2,4-diaminobutyrate decarboxylase	-
BV140_RS05715	1124682..1125080	-	399	WP_005651560.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BV140_RS05720	1125080..1125313	-	234	WP_005686209.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
BV140_RS05725	1125460..1126824	-	1365	WP_005686211.1	diaminobutyrate--2-oxoglutarate transaminase	-
BV140_RS05730	1127171..1127341	-	171	WP_005613503.1	50S ribosomal protein L33	-
BV140_RS05735	1127353..1127589	-	237	WP_005542826.1	50S ribosomal protein L28	-
BV140_RS05740	1127803..1128468	-	666	WP_032821549.1	DNA repair protein RadC	-
BV140_RS05745	1128632..1129834	+	1203	WP_005686217.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15115.46 Da
• Isoelectric Point: 8.0514

>T73747 WP_005651560.1 NZ_CP020013:c1125080-1124682 [Haemophilus influenzae]
VLKYMLDTNIVIYVIKRRPLEILSRFNQNAGKMCVSSITVAELYYGAEKSEYPERNIAVIEDFLSRLTILDYQPKHAAHF
GNIKAELSKQGKLIGENDIHIAAHARSEGLILVSNNLREFERVIALRTENWV

Nucleotide

• Length: 399 bp

>T73747 NZ_CP020013:c1125080-1124682 [Haemophilus influenzae]
GTGTTGAAATATATGCTTGATACTAACATTGTGATTTACGTGATTAAGCGTAGGCCATTGGAAATTCTTTCTCGCTTTAA
TCAAAATGCAGGAAAAATGTGCGTGAGTAGCATTACTGTGGCTGAACTCTATTATGGAGCTGAAAAAAGCGAATATCCCG
AAAGAAACATTGCGGTTATTGAAGATTTCTTATCACGCCTCACCATTTTAGATTACCAACCAAAGCATGCAGCTCATTTC
GGCAATATTAAAGCTGAACTCTCTAAACAAGGGAAATTGATTGGCGAAAATGATATTCATATAGCGGCTCATGCTCGTAG
TGAAGGGTTAATTCTGGTTAGTAACAATTTAAGAGAATTTGAGCGAGTTATAGCATTACGTACAGAAAATTGGGTGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8911.01 Da
• Isoelectric Point: 4.4013

>AT73747 WP_005686209.1 NZ_CP020013:c1125313-1125080 [Haemophilus influenzae]
MIETSVFMTNRSQAVRLPAEVRFSEEIKKLSVRVSGSDRILSPLNQSWDSFFLNDQAVSDDFMNEREIAFQPEREAL

Nucleotide

• Length: 234 bp

>AT73747 NZ_CP020013:c1125313-1125080 [Haemophilus influenzae]
ATGATTGAAACCTCTGTTTTTATGACTAATCGAAGTCAAGCTGTGCGTTTGCCCGCTGAAGTTCGCTTTTCTGAAGAAAT
CAAAAAATTATCGGTTCGTGTATCGGGCAGTGATCGGATTTTATCGCCTCTCAACCAATCTTGGGATAGTTTTTTCTTGA
ATGATCAAGCAGTTAGCGATGATTTTATGAATGAACGAGAAATTGCATTTCAACCAGAGCGTGAGGCTTTATAG

Structures

Toxin

• AlphaFold DB: Q4QLW0