Detailed information of TA system TA073479

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 5880..6305
• Replicon: plasmid unnamed3
• Accession: NZ_CP019909
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: B1200_RS28260
• Protein ID: WP_001332444.1
• Coordinates: 5880..6041 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	6094..6305 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS28225	1230..1457	-	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-
B1200_RS28230	1551..2237	-	687	WP_078081140.1	PAS domain-containing protein	-
B1200_RS28235	2427..2810	-	384	WP_000124981.1	relaxosome protein TraM	-
B1200_RS28240	3144..3734	+	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
B1200_RS28245	4030..4851	-	822	WP_001234485.1	DUF945 domain-containing protein	-
B1200_RS29870	5030..5642	+	613	Protein_8	hypothetical protein	-
B1200_RS28260	5880..6041	-	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	6094..6305	-	212	NuclAT_0	-	Antitoxin
-	6094..6305	-	212	NuclAT_0	-	Antitoxin
-	6094..6305	-	212	NuclAT_0	-	Antitoxin
-	6094..6305	-	212	NuclAT_0	-	Antitoxin
B1200_RS28270	6316..7035	-	720	WP_001276275.1	plasmid SOS inhibition protein A	-
B1200_RS28275	7032..7466	-	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
B1200_RS29875	7521..9479	-	1959	Protein_12	ParB/RepB/Spo0J family partition protein	-
B1200_RS28295	9538..9771	-	234	WP_001209608.1	DUF905 domain-containing protein	-
B1200_RS28300	9834..10331	-	498	WP_050427786.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..85009	85009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T73479 WP_001332444.1 NZ_CP019909:c6041-5880 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T73479 NZ_CP019909:c6041-5880 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 212 bp

>AT73479 NZ_CP019909:c6305-6094 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin