Detailed information of TA system TA073379

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2758069..2758278
• Replicon: chromosome
• Accession: NZ_CP019860
• Organism: Clostridioides difficile strain DSM 29632

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: CDIF29632_RS13125
• Protein ID: WP_004454815.1
• Coordinates: 2758120..2758278 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2758069..2758203 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF29632_RS13110	2753894..2755522	+	1629	WP_009897704.1	aspartate 4-decarboxylase	-
CDIF29632_RS13115	2755643..2756638	+	996	WP_003431031.1	asparaginase	-
CDIF29632_RS13120	2756827..2757210	-	384	Protein_2438	BlaI/MecI/CopY family transcriptional regulator	-
-	2758069..2758203	+	135	NuclAT_2	-	Antitoxin
CDIF29632_RS13125	2758120..2758278	-	159	WP_004454815.1	hypothetical protein	Toxin
CDIF29632_RS13130	2758637..2760007	-	1371	WP_009897709.1	cell wall-binding protein Cwp29	-
CDIF29632_RS13135	2760290..2760388	+	99	Protein_2441	Arm DNA-binding domain-containing protein	-
CDIF29632_RS13140	2760407..2760828	+	422	Protein_2442	hypothetical protein	-
CDIF29632_RS13145	2761059..2761331	+	273	WP_004454820.1	tyrosine-type recombinase/integrase	-
CDIF29632_RS13150	2761649..2762317	-	669	WP_021361938.1	VanZ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T73379 WP_004454815.1 NZ_CP019860:c2758278-2758120 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T73379 NZ_CP019860:c2758278-2758120 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT73379 NZ_CP019860:2758069-2758203 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin