Detailed information of TA system TA073341

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2817759..2817968
• Replicon: chromosome
• Accession: NZ_CP019857
• Organism: Clostridioides difficile strain DSM 29745

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: CDIF29745_RS13610
• Protein ID: WP_004454815.1
• Coordinates: 2817810..2817968 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2817759..2817893 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF29745_RS13595	2813248..2814876	+	1629	WP_009897704.1	aspartate 4-decarboxylase	-
CDIF29745_RS13600	2814997..2815992	+	996	WP_003431031.1	asparaginase	-
CDIF29745_RS13605	2816181..2816564	-	384	Protein_2537	BlaI/MecI/CopY family transcriptional regulator	-
-	2817759..2817893	+	135	NuclAT_0	-	Antitoxin
CDIF29745_RS13610	2817810..2817968	-	159	WP_004454815.1	hypothetical protein	Toxin
CDIF29745_RS13615	2818327..2819697	-	1371	WP_009897709.1	cell wall-binding protein Cwp29	-
CDIF29745_RS13620	2819980..2820078	+	99	Protein_2540	Arm DNA-binding domain-containing protein	-
CDIF29745_RS13625	2820097..2820518	+	422	Protein_2541	hypothetical protein	-
CDIF29745_RS13630	2820749..2821021	+	273	WP_004454820.1	tyrosine-type recombinase/integrase	-
CDIF29745_RS13635	2821384..2821464	-	81	Protein_2543	VOC family protein	-
CDIF29745_RS13640	2821504..2822172	-	669	WP_009897711.1	VanZ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T73341 WP_004454815.1 NZ_CP019857:c2817968-2817810 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T73341 NZ_CP019857:c2817968-2817810 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT73341 NZ_CP019857:2817759-2817893 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin