Detailed information of TA system TA073266

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4905760..4906595
• Replicon: chromosome
• Accession: NZ_CP019777
• Organism: Escherichia coli NU14

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1R2L4
• Locus tag: B0908_RS25175
• Protein ID: WP_001094426.1
• Coordinates: 4906218..4906595 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: B0908_RS25170
• Protein ID: WP_001285575.1
• Coordinates: 4905760..4906128 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0908_RS25135	4901653..4902333	+	681	WP_001282919.1	WYL domain-containing protein	-
B0908_RS25140	4902481..4903158	+	678	WP_001097301.1	hypothetical protein	-
B0908_RS25145	4903164..4903397	+	234	WP_001278290.1	DUF905 family protein	-
B0908_RS25150	4903487..4904305	+	819	WP_001175160.1	DUF945 domain-containing protein	-
B0908_RS25155	4904396..4904881	+	486	WP_000213697.1	antirestriction protein	-
B0908_RS25160	4904896..4905372	+	477	WP_001298074.1	RadC family protein	-
B0908_RS25165	4905459..4905680	+	222	WP_000692350.1	DUF987 domain-containing protein	-
B0908_RS25170	4905760..4906128	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B0908_RS25175	4906218..4906595	+	378	WP_001094426.1	TA system toxin CbtA family protein	Toxin
B0908_RS25180	4906592..4907080	+	489	WP_000761683.1	hypothetical protein	-
B0908_RS25185	4907092..4907289	+	198	WP_000839272.1	DUF957 domain-containing protein	-
B0908_RS26570	4907368..4907526	+	159	WP_001467148.1	hypothetical protein	-
B0908_RS25195	4908538..4910514	+	1977	WP_000464678.1	hypothetical protein	-
B0908_RS25200	4910913..4911092	-	180	WP_001298063.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	4893057..4920651	27594

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.99 Da
• Isoelectric Point: 7.3523

>T73266 WP_001094426.1 NZ_CP019777:4906218-4906595 [Escherichia coli NU14]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T73266 NZ_CP019777:4906218-4906595 [Escherichia coli NU14]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT73266 WP_001285575.1 NZ_CP019777:4905760-4906128 [Escherichia coli NU14]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT73266 NZ_CP019777:4905760-4906128 [Escherichia coli NU14]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454ABD6

Antitoxin

• AlphaFold DB: A0A454ABF5