Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3229958..3230612 Replicon chromosome
Accession NZ_CP019777
Organism Escherichia coli NU14

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag B0908_RS16635 Protein ID WP_000244781.1
Coordinates 3229958..3230365 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag B0908_RS16640 Protein ID WP_000354046.1
Coordinates 3230346..3230612 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0908_RS16615 3225915..3227648 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
B0908_RS16620 3227654..3228364 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
B0908_RS16625 3228389..3229285 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
B0908_RS16630 3229397..3229918 + 522 WP_001055874.1 flavodoxin FldB -
B0908_RS16635 3229958..3230365 - 408 WP_000244781.1 protein YgfX Toxin
B0908_RS16640 3230346..3230612 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
B0908_RS16645 3230855..3231835 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
B0908_RS16650 3231912..3232571 - 660 WP_000250274.1 hemolysin III family protein -
B0908_RS16655 3232735..3233046 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
B0908_RS16660 3233091..3234524 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T73263 WP_000244781.1 NZ_CP019777:c3230365-3229958 [Escherichia coli NU14]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T73263 NZ_CP019777:c3230365-3229958 [Escherichia coli NU14]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT73263 WP_000354046.1 NZ_CP019777:c3230612-3230346 [Escherichia coli NU14]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT73263 NZ_CP019777:c3230612-3230346 [Escherichia coli NU14]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References